The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool.
The active microbial community more accurately reflects the anaerobic digestion process: 16S rRNA (gene) sequencing as a predictive tool.
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DOI：
10.1186/s40168-018-0449-9
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发表时间：
2018-04-02
期刊：
影响因子：
15.5
通讯作者：
Ijaz UZ
中科院分区：
文献类型：
--
作者：
De Vrieze J;Pinto AJ;Sloan WT;Ijaz UZ
针对16S rRNA基因的扩增子测序方法已广泛用于研究厌氧消化中的微生物群落组成和动态。这些方法成功地表征了扩增子，但不能区分实际负责该过程的微生物。本研究通过16S rRNA（基因）扩增子测序，对48株全规模厌氧消化植物的古细菌和细菌群落进行了DNA（总群落）和RNA（活性群落）水平的评价。与RNA水平相比，古细菌的DNA多样性显著高于RNA水平，而细菌则不然。β多样性分析表明，细菌和古细菌的DNA和RNA在群落组成上存在显著差异。这与细菌和古菌的总OTUs分别占25.5%和42.3%有关，这表明它们的DNA和RNA谱存在显著差异。相似的操作参数影响了细菌和古菌群落，但古菌对DNA和RNA的区分作用要强得多。共现网络和功能预测谱证实了DNA和RNA谱之间的明显差异。总之，活性（RNA）和总（DNA）群落谱存在明显差异，这意味着需要一种综合方法来评估厌氧消化中的群落稳定性。本文的在线版本（10.1186/s40168-018-0449-9）包含补充材料，可供授权用户使用。
Amplicon sequencing methods targeting the 16S rRNA gene have been used extensively to investigate microbial community composition and dynamics in anaerobic digestion. These methods successfully characterize amplicons but do not distinguish micro-organisms that are actually responsible for the process. In this research, the archaeal and bacterial community of 48 full-scale anaerobic digestion plants were evaluated on DNA (total community) and RNA (active community) level via 16S rRNA (gene) amplicon sequencing. A significantly higher diversity on DNA compared with the RNA level was observed for archaea, but not for bacteria. Beta diversity analysis showed a significant difference in community composition between the DNA and RNA of both bacteria and archaea. This related with 25.5 and 42.3% of total OTUs for bacteria and archaea, respectively, that showed a significant difference in their DNA and RNA profiles. Similar operational parameters affected the bacterial and archaeal community, yet the differentiating effect between DNA and RNA was much stronger for archaea. Co-occurrence networks and functional prediction profiling confirmed the clear differentiation between DNA and RNA profiles. In conclusion, a clear difference in active (RNA) and total (DNA) community profiles was observed, implying the need for a combined approach to estimate community stability in anaerobic digestion. The online version of this article (10.1186/s40168-018-0449-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1007/s11157-008-9131-1
发表时间：
2008-06-01
期刊：
Reviews in Environmental Science and Bio/Technology
影响因子：
--
作者：
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通讯作者：
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影响因子：
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影响因子：
11
作者：
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