Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent.
Diversity and population structure of sewage-derived microorganisms in wastewater treatment plant influent.
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DOI：
10.1111/j.1462-2920.2009.02075.x
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发表时间：
2010-02
影响因子：
5.1
通讯作者：
Sogin ML
中科院分区：
文献类型：
--
作者：
McLellan SL;Huse SM;Mueller-Spitz SR;Andreishcheva EN;Sogin ML
未经处理的污水的排放将不确定组成的非本地微生物种群引入地表水。我们使用rRNA基因高变区大规模平行454测序来分析密尔沃基大都会两个污水处理厂（WWTP）的8个未经处理的污水流入样本的微生物群落。污水剖面包括一个可识别的人类粪便特征，由几个分类群组成，包括多个双歧杆菌科、毛杆菌科、拟杆菌科、毛杆菌科和瘤胃球菌科属。粪便特征只占污水中存在的分类群的一小部分，但这些序列标签的相对丰度反映了人类粪便样本的种群结构。这些属在污水中比标准指示种普遍得多。来自β -和γ变形菌属分类群的高丰度序列在污水样品中占主导地位，但在粪便和地表水样品中出现的水平非常低，表明这些生物在下水道系统中增殖。来自琼斯岛污水处理厂（JI -服务于住宅和联合污水处理系统）和南岸污水处理厂（SS -服务于住宅区）的样本具有非常一致的社区概况，在给定收集日的污水处理厂之间的相似性比在不同日期收集的同一工厂更大。降雨增加了SS和JI污水处理厂的进水流量，这对应于两个工厂更大的群落多样性。总的来说，下水道系统似乎是一个明确的环境，雨水的渗透和雨水的输入调节着社区的组成。微生物污水群落代表了人类粪便微生物输入和环境中特定微生物富集的组合，形成了独特的种群结构。
The release of untreated sewage introduces non-indigenous microbial populations of uncertain composition into surface waters. We used massively parallel 454 sequencing of hypervariable regions in rRNA genes to profile microbial communities from eight untreated sewage influent samples of two wastewater treatment plants (WWTP) in metropolitan Milwaukee. The sewage profiles included a discernable human fecal signature made up of several taxonomic groups including multiple Bifidobacteriaceae, Coriobacteriaceae, Bacteroidaceae, Lachnospiraceae, and Ruminococcaceae genera. The fecal signature made up a small fraction of the taxa present in sewage but the relative abundance of these sequence tags mirrored the population structures of human fecal samples. These genera were much more prevalent in the sewage influent than standard indicators species. High-abundance sequences from taxonomic groups within the Beta- and Gammaproteobacteria dominated the sewage samples but occurred at very low levels in fecal and surface water samples, suggesting that these organisms proliferate within the sewer system. Samples from Jones Island (JI – servicing residential plus a combined sewer system) and South Shore (SS – servicing a residential area) WWTPs had very consistent community profiles, with greater similarity between WWTPs on a given collection day than the same plant collected on different days. Rainfall increased influent flows at SS and JI WWTPs, and this corresponded to greater diversity in the community at both plants. Overall, the sewer system appears to be a defined environment with both infiltration of rainwater and stormwater inputs modulating community composition. Microbial sewage communities represent a combination of inputs from human fecal microbes and enrichment of specific microbes from the environment to form a unique population structure.
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影响因子：
4.4
作者：
Bernhard, AE;Field, KG
通讯作者：
Field, KG
影响因子：
3
作者：
Field, KG;Bernhard, AE;Brodeur, TJ
通讯作者：
Brodeur, TJ
影响因子：
12.8
作者：
Chen, GH;Leung, DHW;Hung, JC
通讯作者：
Hung, JC
影响因子：
4.4
作者：
Dick, LK;Bernhard, AE;Field, KG
通讯作者：
Field, KG
DOI：
10.1073/pnas.0706625104
发表时间：
2007-08-21
影响因子：
11.1
作者：
Frank, Daniel N.;Amand, Allison L. St.;Pace, Norman R.
通讯作者：
Pace, Norman R.