Inhibin mRNAs in ovine and bovine ovarian follicles and corpora lutea throughout the estrous cycle and gestation

Inhibin mRNAs in ovine and bovine ovarian follicles and corpora lutea throughout the estrous cycle and gestation
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整个动情周期和妊娠期间绵羊和牛卵泡和黄体中的抑制素 mRNA

DOI:
10.1016/0303-7207(89)90117-2
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发表时间:
1989
影响因子:
4.1
通讯作者:
J. Findlay
J. Findlay
中科院分区:
医学2区
文献类型:
--
作者:
R. Rodgers;S. Stuchbery;J. Findlay

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从绵羊和牛卵巢中解剖卵泡和黄体,提取 RNA 并使用牛 cDNA 和 cRNA 探针对 α- 和 βA- 抑制素 mRNA 进行 Northern 印迹分析。使用检测胆固醇侧链裂解细胞色素P-450 (P-450scc) mRNA的cDNA探针作为阳性对照。在牛身上,卵巢基质中未检测到 α- 和 βA- 抑制素 mRNA,而卵巢基质中可能含有直径达 0.5 毫米的卵泡。在牛窦卵泡中检测到抑制素α-和β-AmRNA,但在排卵后,α-和βA-抑制素mRNA的相对水平下降,并且在成熟完全发育的循环黄体以及从妊娠早期到妊娠晚期的妊娠黄体中检测不到α-和βA-抑制素mRNA。在绵羊的一组窦卵泡中检测到了 α- 和 βA- 抑制素 mRNA,但在周期或妊娠黄体中未检测到,后者确实含有 P-450sccmRNA。结论:在牛、羊中,卵泡和未成熟的黄体是抑制素的卵巢来源。
Follicles and corpora lutea were dissected from ovine and bovine ovaries and the RNA extracted and subjected to Northern blot analyses forα- andβA-inhibin mRNAs, using bovine cDNA and cRNA probes. A cDNA probe detecting mRNA for cholesterol side-chain cleavage cytochromeP-450 (P-450scc) was used as a positive control. In cattle,α- andβA-inhibin mRNAs were not detected in ovarian stroma, which could potentially have contained follicles up to 0.5 mm in diameter. Inhibinα- andβ-AmRNAs were detected in bovine antral follicles but after ovulation, the relative levels ofα- andβA-inhibin mRNAs declined and were undetectable in mature fully developed cyclic corpora lutea and in pregnancy corpora lutea from early to late gestation of the cow. In sheep,α- andβA-inhibin mRNAs were detected in a pool of antral follicles but not in cyclic or pregnancy corpora lutea, which did containP-450sccmRNA. It is concluded that in cattle and sheep, follicles and not mature corpora lutea are the ovarian source of inhibin.
DOI: 10.1210/mend-1-11-849
发表时间: 1987-11-01
影响因子: --
作者:
ESCH, FS;SHIMASAKI, S;GUILLEMIN, R
通讯作者: GUILLEMIN, R
生殖周期期间大鼠卵泡中抑制素信使 RNA 的动态变化。
DOI: 10.1126/science.3125611
发表时间: 1988
期刊: Science (New York, N.Y.)
影响因子: --
作者:
Woodruff,TK;D'Agostino,J;Schwartz,NB;Mayo,KE
通讯作者: Mayo,KE
在整个卵巢周期中,牛卵泡和黄体中编码胆固醇侧链裂解细胞色素 P-450、17 α-羟化酶细胞色素 P-450、肾上腺素和低密度脂蛋白受体的信使核糖核酸水平。
DOI: 10.1210/mend-1-3-274
发表时间: 1987
期刊: Molecular endocrinology (Baltimore, Md.)
影响因子: --
作者:
Rodgers,RJ;Waterman,MR;Simpson,ER
通讯作者: Simpson,ER