The role of CORM-2 as a modulator of oxidative stress and hemostatic parameters of human plasma in vitro.
The role of CORM-2 as a modulator of oxidative stress and hemostatic parameters of human plasma in vitro.
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DOI：
10.1371/journal.pone.0184787
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Olas B
中科院分区：
文献类型：
--
作者：
Adach W;Olas B
本实验的主要目的是研究一氧化碳（CO）供体CORM-2对体外人血浆氧化应激的影响。此外，它还检查了CORM-2对血浆止血参数的影响：活化部分凝血活素时间（APTT），凝血酶时间（TT）和凝血酶原时间（PT）。人血浆与不同浓度的CORM-2: 0.1-100 μM孵育5-60 min。随后，研究了各种止血因子和氧化应激的生物标志物。脂质过氧化用硫代巴比妥酸反应物质（TBARS）浓度来测定，蛋白质中氨基酸残基的氧化用羰基和巯基的量来测定。采用过氧化氢（H2O2）和羟基自由基供体（H2O2/Fe）两种氧化应激诱导剂。在测试的CORM-2浓度下，检测到蛋白质羰基化，巯基氧化和脂质过氧化的减少。我们的研究结果表明，CORM-2可能在H2O2或H2O2/Fe处理的人血浆中具有抗氧化特性。此外，我们的研究结果表明CORM-2在体外具有抗凝血活性。
The main aim of the experiment is to examine the effect of CORM-2, a donor of carbon monoxide (CO), on oxidative stress in human plasma in vitro. In addition, it examines the effects of CORM-2 on the hemostatic parameters of plasma: the activated partial thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT). Human plasma was incubated for 5–60 min with different concentrations of CORM-2: 0.1–100 μM. Following this, various hemostatic factors and biomarkers of oxidative stress were studied. Lipid peroxidation was measured as thiobarbituric acid reactive substance (TBARS) concentration, and the oxidation of amino acid residues in proteins was measured by determining the amounts of carbonyl and thiol groups. Two oxidative stress inducers: hydrogen peroxide (H2O2) and the donor of hydroxyl radical (H2O2/Fe) were used. Decrease in protein carbonylation, thiol group oxidation and lipid peroxidation were detected at tested concentrations of CORM-2. Our results indicate that CORM-2 may have antioxidant properties in human plasma treated with H2O2 or H2O2/Fe. In addition, our results indicate the anti-coagulant activities of CORM-2 in vitro.
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