Sialylated glycoconjugates in chromophobe cell renal carcinoma compared with other renal cell tumors
Sialylated glycoconjugates in chromophobe cell renal carcinoma compared with other renal cell tumors
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嫌色细胞肾癌与其他肾细胞肿瘤中唾液酸化糖缀合物的比较
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
R. Fischer
中科院分区：
文献类型：
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作者：
M. Ortmann;M. Vierbuchen;R. Fischer
摘要本研究旨在揭示通过 Hale 胶体铁反应检测到的嫌色细胞肾癌的非凡组织化学特性。特别强调细胞质糖复合物的凝集素组织化学分析。酶促释放唾液酸后花生凝集素 (PNA) 和刺桐凝集素 (ECA) 的结合以及白豆凝集素 (DBA) 的直接结合与接骨木凝集素 (SNA) 和山槐凝集素 (MAA) 的结合位点的表达密切相关，揭示了其中丰富的唾液酸化碳水化合物部分细胞质。这种特征性结合模式与大多数其他肾癌中观察到的微弱染色有很大不同，从而证实嫌色细胞肾癌是一个独特的实体。然而，肾嗜酸细胞瘤的凝集素结合模式明显类似于嫌色细胞癌，表明这些肾肿瘤之间的密切关系。对邻近肾实质的详细分析显示，凝集素结合模式与嫌色癌中描述的非常相似，仅存在于集合管内壁的嵌入细胞中。这一发现表明嫌色细胞肾癌起源于集合管上皮。检测到由改变的上皮组成的小复合体，其显示嫌色癌的形态学特征和嵌入细胞的组织化学特性，可能表明在嫌色癌发展之前出现肿瘤前病变。尽管显然需要进一步的研究来阐明检测到的细胞糖缀合物的生理作用，但目前的结果已经为嫌色细胞肾癌的组织发生和发病机制提供了有价值的见解。
SummaryThe present study was designed to shed light on the extraordinary histochemical properties of the chromophobe cell renal carcinoma detected by Hale’s colloidal iron reaction. Special emphasis was laid on the lectin histochemical analysis of cytoplasmic glycoconju-gates. Binding of peanut agglutinin (PNA) and Erythrina cristagalli agglutinin (ECA) after enzymatic release of sialic acid and direct binding of Dolichos biflorus agglutinin (DBA) correlates well with the expression of binding sites for Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA) revealing abundant sialylated carbohydrate moieties within the cytoplasm. This characteristic binding pattern differs considerably from the faint staining observed in the majority of other renal carcinomas, thus confirming that the chromophobe cell renal carcinoma is a distinct entity. However, the lectin binding pattern of renal oncocytoma obviously resembles that of chromophobe carcinoma indicating a close relationship between these renal tumors. Detailed analysis of adjacent renal parenchyma revealed a lectin binding pattern quite similar to that described in the chromophobe carcinomas exclusively in the intercalated cells lining the collecting duct. This finding suggests that the chromophobe cell renal carcinoma originates from the collecting duct epithelium. The detection of small complexes consisting of altered epithelia which display the morphological characteristics of chromophobe carcinoma and the histochemical properties of intercalated cells probably indicates the emergence of preneoplastic lesions preceding the development of chromophobe carcinoma. Even though further studies are clearly needed to elucidate the physiological role of the cellular glycoconjugates detected, the present results already provide valuable insight into the histogenesis and pathogenesis of the chromophobe cell renal carcinoma.
大鼠外髓集合管闰细胞对慢性代谢性酸中毒的反应。
DOI：
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发表时间：
1984
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
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作者：
Madsen,KM;Tisher,CC
通讯作者：
Tisher,CC
DOI：
10.1016/s0021-9258(19)75677-4
发表时间：
1987-02
期刊：
The Journal of biological chemistry
影响因子：
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作者：
N. Shibuya;I. Goldstein;W. Broekaert;M. Nsimba-Lubaki;B. Peeters;W. Peumans
通讯作者：
N. Shibuya;I. Goldstein;W. Broekaert;M. Nsimba-Lubaki;B. Peeters;W. Peumans
花生凝集素与肾脏中特定上皮细胞类型的结合。
DOI：
10.1152/ajpcell.1982.242.1.c117
发表时间：
1982
期刊：
The American journal of physiology
影响因子：
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作者：
LeHir,M;Kaissling,B;Koeppen,BM;Wade,JB
通讯作者：
Wade,JB