CFU-S assay: a historical single-cell assay that offers modern insight into clonal hematopoiesis.
CFU-S assay: a historical single-cell assay that offers modern insight into clonal hematopoiesis.
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DOI：
10.1016/j.exphem.2021.10.003
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发表时间：
2021-12
影响因子：
2.6
通讯作者：
Camilla Forsberg, E.
中科院分区：
文献类型：
--
作者：
Rodriguez y Baena, Alessandra;Manso, Bryce A.;Camilla Forsberg, E.
自1961年James Till博士和Ernest McCulloch博士开发了第一个体内克隆策略（称为脾集落形成单位（CFU-S）测定）以在单细胞水平评估骨髓来源的造血祖细胞的功能能力以来，造血干细胞（HSC）已被广泛研究。通过移植骨髓细胞和分析脾中产生的细胞结节，CFU-S测定揭示了造血祖细胞的自我更新和克隆分化能力。该测定的进一步开发和使用已经鉴定出具有克隆、多谱系分化的高度增殖、自我更新和分化的HSC。CFU-S策略也已被调整为询问单个纯化的造血干细胞和祖细胞群体，推进了我们对造血层次的认识。在这篇综述中，我们探讨了CFU-S检测的主要发现，考虑其现代应用和最近的改进，并将其与常用的长期移植检测进行比较，以确定CFU-S检测对理解HSC生物学和造血的持续价值。
Hematopoietic stem cells (HSCs) have been studied extensively since their initial functional description in 1961 when Dr. James Till and Dr. Ernest McCulloch developed the first in vivo clonal strategy, termed the spleen colony-forming unit (CFU-S) assay, to assess the functional capacity of bone marrow–derived hematopoietic progenitors at the single-cell level. Through transplantation of bone marrow cells and analysis of the resulting cellular nodules in the spleen, the CFU-S assay revealed both the self-renewal and clonal differentiation capacity of hematopoietic progenitors. Further development and use of this assay have identified highly proliferative, self-renewing, and differentiating HSCs that possess clonal, multilineage differentiation. The CFU-S strategy has also been adapted to interrogating single purified hematopoietic stem and progenitor cell populations, advancing our knowledge of the hematopoietic hierarchy. In this review, we explore the major discoveries made with the CFU-S assay, consider its modern use and recent improvements, and compare it with commonly used long-term transplantation assays to determine the continued value of the CFU-S assay for understanding HSC biology and hematopoiesis.
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克隆组装！个体发育和疾病期间血液的克隆复杂性。
影响因子：
2.6
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
LOUTIT, JF
DOI：
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发表时间：
1986-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
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作者：
MULLIS, K;FALOONA, F;ERLICH, H
通讯作者：
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DOI：
10.1073/pnas.0906556106
发表时间：
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影响因子：
11.1
作者：
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通讯作者：
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