Heparanase localization during palatogenesis in mice.
Heparanase localization during palatogenesis in mice.
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DOI：
10.1155/2013/760236
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发表时间：
2013
影响因子：
--
通讯作者：
Nakamura H
中科院分区：
文献类型：
--
作者：
Hirata A;Katayama K;Tsuji T;Natsume N;Sugahara T;Koga Y;Takano K;Otsuki Y;Nakamura H
腭突的发生是由上皮-间充质相互作用决定的，部分是腭突细胞外基质（ECM）重塑的结果。在这里，我们评估了乙酰肝素酶在发育中的小鼠腭的分布。在腭形成的早期阶段，在垂直方向的腭架中没有观察到乙酰肝素酶。随着腭形成的进展，腭架被重组，并在舌上方水平排列，乙酰肝素酶定位于这些架的上皮细胞。当腭两侧的货架第一次接触，乙酰肝素酶定位于上皮细胞在货架的提示。随后在融合腭架时，内侧上皮缝（MES）的细胞被强烈的乙酰肝素酶信号标记。相反，基底膜硫酸乙酰肝素（HS）几乎没有观察到在腭架接触。此外，串珠素标记稀疏的基底膜的MES，层粘连蛋白和IV型胶原。此外，我们评估了基质金属蛋白酶-（MMP-）9，MMP-2和MMP-3在发育中的小鼠腭和这些MMPs在MES中观察到的分布。我们的研究结果表明，乙酰肝素酶是重要的腭形成，因为它介导的腭架ECM的降解。乙酰肝素酶可能与其他蛋白酶一起参与MES的消退。
Palatogenesis is directed by epithelial-mesenchymal interactions and results partly from remodeling of the extracellular matrix (ECM) of the palatal shelves. Here, we assessed heparanase distribution in developing mouse palates. No heparanase was observed in the vertically oriented palatal shelves in early stages of palate formation. As palate formation progressed, the palatal shelves were reorganized and arranged horizontally above the tongue, and heparanase localized to the epithelial cells of these shelves. When the palatal bilateral shelves first made contact, the heparanase localized to epithelial cells at the tips of shelves. Later in fusing palatal shelves, the cells of the medial epithelial seam (MES) were labeled with intense heparanase signal. In contrast, the basement membrane heparan sulfate (HS) was scarcely observed in the palatal shelves in contact. Moreover, perlecan labeling was sparse in the basement membrane of the MES, on which laminin and type IV collagen were observed. Moreover, we assessed the distribution of matrix metalloproteinase- (MMP-) 9, MMP-2, and MMP-3 in developing mouse palates and these MMPs were observed in the MES. Our findings indicated that heparanase was important for palate formation because it mediated degradation of the ECM of palatal shelves. Heparanase may, in concert with other proteases, participate in the regression of the MES.
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影响因子：
6.5
作者：
Bernard, D;Méhul, B;Schmidt, R
通讯作者：
Schmidt, R
DOI：
10.1016/j.biocel.2005.03.006
发表时间：
2005-09-01
影响因子：
4
作者：
Cool, SM;Nurcombe, V
通讯作者：
Nurcombe, V
影响因子：
4.6
作者：
Cuervo, R;Covarrubias, L
通讯作者：
Covarrubias, L
影响因子：
3.3
作者：
Blavier, L;Lazaryev, A;Kaartinen, V
通讯作者：
Kaartinen, V
影响因子：
5.5
作者：
Khan, Shaukat A.;Nelson, Matthew S.;Gupta, Pankaj
通讯作者：
Gupta, Pankaj