Quantitation of Tolyporphins, Diverse Tetrapyrrole Secondary Metabolites with Chlorophyll-Like Absorption, from a Filamentous Cyanobacterium-Microbial Community.
Quantitation of Tolyporphins, Diverse Tetrapyrrole Secondary Metabolites with Chlorophyll-Like Absorption, from a Filamentous Cyanobacterium-Microbial Community.
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对来自丝状蓝藻-微生物群落的 Tolyporphins（具有叶绿素样吸收的多种四吡咯次级代谢物）进行定量。
DOI：
10.1002/pca.2735
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Lindsey,JonathanS
中科院分区：
文献类型：
--
作者：
Zhang,Yunlong;Zhang,Ran;Hughes,Rebecca-Ayme;Dai,Jingqiu;Gurr,JoshuaR;Williams,PhilipG;Miller,EricS;Lindsey,JonathanS
Tolyporphins是由一种非纯性丝状蓝藻（HT-58 - 2）产生的不常见的四吡咯大环化合物。Tolyporphins A-J、L和M共享共同的二氧菌绿素核心，在外围取代基上不同，并且表现出与占主导地位的蓝藻色素叶绿素重叠的吸收光谱。鉴定和准确定量的各种tolyporphins在这些叶绿素丰富的samples提出了challenges. ObjectiveDevelopmentmethods的tolyporphins在各种生长条件下产生的定量测定相对于chlorophylla. MethodologyChromatographicfractionationoflarge ‐scale（440 L）文化提供了独立的tolyporphins。小规模（25 mL）培养物的亲脂性提取、使用内标物的HPLC分离和吸收检测能够定量甲苯卟啉A和叶绿素，并通过推断定量甲苯卟啉A-M的量。吸收光谱与亲脂性提取物（2 mL培养物）的多组分分析提供了所有tolyporphins叶绿素的比例。报道的吸收光谱数据的各种tolyporphins需要重新评估定量purposes.Results和DiscussionThe量tolyporphin A后50天的光照范围从0.13 nmol/mg干细胞（含硝酸盐的介质）到1.12 nmol/mg（无硝酸盐），最大的0.23倍，叶绿素。在可溶性氮剥夺35-50天后，tolyporphin A代表总tolyporphins的1/3-1/2，tolyporphins的总量是chlorophylla. Conclusions本文开发的定量方法应有利于tolyporphins（和其他tetrapyrroles）的生物合成的调查，以及其他菌株生产tolyporphins的检查。版权所有© 2017约翰威利父子有限公司
IntroductionTolyporphins are unusual tetrapyrrole macrocycles produced by a non‐axenic filamentous cyanobacterium (HT‐58‐2). Tolyporphins A–J, L, and M share a common dioxobacteriochlorin core, differ in peripheral substituents, and exhibit absorption spectra that overlap that of the dominant cyanobacterial pigment, chlorophylla. Identification and accurate quantitation of the various tolyporphins in these chlorophyll‐rich samples presents challenges.ObjectiveTo develop methods for the quantitative determination of tolyporphins produced under various growth conditions relative to that of chlorophylla.MethodologyChromatographic fractionation of large‐scale (440 L) cultures afforded isolated individual tolyporphins. Lipophilic extraction of small‐scale (25 mL) cultures, HPLC separation with an internal standard, and absorption detection enabled quantitation of tolyporphin A and chlorophylla, and by inference the amounts of tolyporphins A–M. Absorption spectroscopy with multicomponent analysis of lipophilic extracts (2 mL cultures) afforded the ratio of all tolyporphins to chlorophylla. The reported absorption spectral data for the various tolyporphins required re‐evaluation for quantitative purposes.Results and DiscussionThe amount of tolyporphin A after 50 days of illumination ranged from 0.13 nmol/mg dry cells (media containing nitrate) to 1.12 nmol/mg (without nitrate), with maximum 0.23 times that of chlorophylla. Under soluble‐nitrogen deprivation after 35–50 days, tolyporphin A represents 1/3–1/2 of the total tolyporphins, and the total amount of tolyporphins is up to 1.8‐fold that of chlorophylla.ConclusionsThe quantitative methods developed herein should facilitate investigation of the biosynthesis of tolyporphins (and other tetrapyrroles) as well as examination of other strains for production of tolyporphins. Copyright © 2017 John Wiley & Sons, Ltd.
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