Localization and androgen regulation of metastasis-associated protein 1 in mouse epididymis.
Localization and androgen regulation of metastasis-associated protein 1 in mouse epididymis.
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小鼠附睾中转移相关蛋白 1 的定位和雄激素调节
DOI：
10.1371/journal.pone.0015439
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发表时间：
2010-11-03
期刊：
影响因子：
3.7
通讯作者：
Zhang YQ
中科院分区：
文献类型：
--
作者：
Ma L;Li W;Zhu HP;Li Z;Sun ZJ;Liu XP;Zhao J;Zhang JS;Zhang YQ
转移相关蛋白1（MTA 1）是MTA基因家族的创始成员，可以通过影响染色质重塑状态来调节转录。尽管它与癌细胞的转移潜力有很强的相关性，但MTA 1也可以通过改变乙酰化状态来调节关键的细胞通路。我们以前曾报道过MTA 1/MTA 1在人类和小鼠睾丸中的存在，提供了证据，其参与调节小鼠精子发生过程中的睾丸功能。本研究的目的是进一步从转录和翻译水平研究MTA 1在小鼠附睾中的定位，并探讨MTA 1的表达是否受雄激素和生后附睾发育的调控。通过双侧去势剥夺小鼠循环雄激素，然后补充外源性丙酸睾酮一周。MTA 1免疫定位于整个附睾上皮，在近端区域的主细胞和透明细胞的细胞核中表达最多。去势后其表达逐渐下降，而睾酮处理可恢复其表达，表明该基因的表达依赖于雄激素。在出生后发育过程中，附睾中的蛋白质表达开始出现从出生后第7天到第14天，从出生后第28天急剧增加，并在成年后达到峰值，与附睾的分化良好的状态和循环雄激素的成熟水平相一致。这种区域和细胞特异性模式在正常人附睾中也是保守的。结果表明，MTA 1蛋白的表达受雄激素途径的调控，其表达水平与附睾的发育密切相关，提示该基因可能在精子成熟和生育中起重要作用。
Metastasis-associated protein 1 (MTA1), the founding member of the MTA family of genes, can modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of cancer cells, MTA1 can also regulate crucial cellular pathways by modifying the acetylation status. We have previously reported the presence of MTA1/MTA1 in human and mouse testes, providing the evidence for its involvement in the regulation of testicular function during murine spermatogenesis. The objective of present study was to further assess the localization of MTA1 in mouse epididymis on both transcriptional and translational level, and then to explore whether MTA1 expression is regulated by androgens and postnatal epididymal development. Mice were deprived of circulating androgen by bilaterally castration and were then supplemented with exogenous testosterone propionate for one week. MTA1 was immunolocalized in the epithelium of the entire epididymis with the maximal expression in the nuclei of principal cells and of clear cells in proximal region. Its expression decreased gradually after castration, whereas testosterone treatment could restore the expression, indicating that the expression of this gene is dependent on androgen. During postnatal development, the protein expression in the epididymis began to appear from day 7 to day 14, increased dramatically from postnatal day 28, and peaked at adulthood onwards, coinciding with both the well differentiated status of epididymis and the mature levels of circulating androgens. This region- and cell-specific pattern was also conservative in normal human epididymis. Our data suggest that the expression of MTA1 protein could be regulated by androgen pathway and its expression level is closely associated with the postnatal development of the epididymis, giving rise to the possibility that this gene plays a potential role in sperm maturation and fertility.
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作者：
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通讯作者：
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