Comparison of the efficiency of Banna miniature inbred pig somatic cell nuclear transfer among different donor cells.
Comparison of the efficiency of Banna miniature inbred pig somatic cell nuclear transfer among different donor cells.
复制标题
版纳小型自交猪不同供体细胞体细胞核移植效率比较
DOI：
10.1371/journal.pone.0057728
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zeng Y
中科院分区：
文献类型：
--
作者：
Wei H;Qing Y;Pan W;Zhao H;Li H;Cheng W;Zhao L;Xu C;Li H;Li S;Ye L;Wei T;Li X;Fu G;Li W;Xin J;Zeng Y
体细胞核移植是培育优质品种、扩大种群、保护濒危物种的重要手段。然而，SCNT胚胎的生存能力较差，在猪身上的动物生产克隆率较低。本研究旨在研究版纳小型近交系猪的基因功能，建立疾病模型。用来自胎儿、新生儿和成人成纤维细胞的供体细胞进行了SCNT，并比较了供体细胞的克隆效率。结果表明，以版纳小型近交猪胎儿成纤维细胞构建的胚胎（74.3%和27.4%）与新生儿成纤维细胞构建的胚胎（76.4%和21.8%）的卵裂率和囊胚形成率无显著差异（P < 0.05）。但胎儿和新生儿成纤维细胞组均显著高于成人成纤维细胞组（61.9%和13.0%，P<0.05）。胎儿和新生儿成纤维细胞组受体的妊娠率（分别为60%和80%）高于成人成纤维细胞组。分别从胎儿、新生儿和成年成纤维细胞的重建胚胎中获得了8只、3只和1只克隆仔猪。微卫星分析结果表明，所有克隆仔猪的基因型均与其供体细胞相同，且版纳小型近交系猪的遗传纯合度高于受体。因此，利用版纳小型近交系猪的胎儿、新生儿和成年成纤维细胞作为供体细胞，成功克隆了后代。
Somatic cell nuclear transfer (SCNT) is an important method of breeding quality varieties, expanding groups, and preserving endangered species. However, the viability of SCNT embryos is poor, and the cloned rate of animal production is low in pig. This study aims to investigate the gene function and establish a disease model of Banna miniature inbred pig. SCNT with donor cells derived from fetal, newborn, and adult fibroblasts was performed, and the cloning efficiencies among the donor cells were compared. The results showed that the cleavage and blastocyst formation rates did not significantly differ between the reconstructed embryos derived from the fetal (74.3% and 27.4%) and newborn (76.4% and 21.8%) fibroblasts of the Banna miniature inbred pig (P>0.05). However, both fetal and newborn fibroblast groups showed significantly higher rates than the adult fibroblast group (61.9% and 13.0%; P<0.05). The pregnancy rates of the recipients in the fetal and newborn fibroblast groups (60% and 80%, respectively) were higher than those in the adult fibroblast group. Eight, three, and one cloned piglet were obtained from reconstructed embryos of the fetal, newborn, and adult fibroblasts, respectively. Microsatellite analyses results indicated that the genotypes of all cloning piglets were identical to their donor cells and that the genetic homozygosity of the Banna miniature inbred pig was higher than those of the recipients. Therefore, the offspring was successfully cloned using the fetal, newborn, and adult fibroblasts of Banna miniature inbred pig as donor cells.
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影响因子：
1.8
作者：
Hosseini, S. M.;Moulavi, F.;Nasr-Esfahani, M. H.
通讯作者：
Nasr-Esfahani, M. H.
影响因子：
1.8
作者：
Kurome, Mayuko;Ishikawa, Takayuki;Nagashima, Hiroshi
通讯作者：
Nagashima, Hiroshi
影响因子：
3.6
作者：
Inoue, K;Ogonuki, N;Ogura, A
通讯作者：
Ogura, A
影响因子：
2.8
作者：
Lee, GS;Hyun, SH;Hwang, WS
通讯作者：
Hwang, WS
影响因子：
2.8
作者：
Martin, M;Adams, C;Wiseman, B
通讯作者：
Wiseman, B