Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NF-kB.
Wnt1 Participates in Inflammation Induced by Lipopolysaccharide Through Upregulating Scavenger Receptor A and NF-kB.
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Wnt1 通过上调清道夫受体 A 和 NF-kB 参与脂多糖诱导的炎症。
DOI：
10.1007/s10753-015-0147-8
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发表时间：
2015-08
期刊：
影响因子：
5.1
通讯作者：
Zheng, Liangrong
中科院分区：
文献类型：
--
作者：
Zhao, Wenting;Sun, Zewei;Wang, Shuai;Li, Zhenwei;Zheng, Liangrong
该研究调查了wnt1在脂多糖（LPS）引发的炎症反应中的作用，并分析了wnt1、NF-KB和炎症因子之间的关联。 THP-1 细胞用 phorbol-12-myristate-13-acetate (PMA) 激活，并用 LPS 处理以诱导炎症。用wnt1siRNA和过表达质粒转染THP-1细胞，探讨wnt1、SRA和NF-KB之间的关系。使用β-catenin抑制剂和FZD1的siRNA来研究wnt1诱导的SRA激活中涉及的信号转导事件。在 wnt1 siRNA 和 LPS 治疗后评估 NF-kB 蛋白和炎症细胞因子的水平。 THP-1 细胞的 PMA 激活和 LPS 处理增加了 wnt1 蛋白水平。 Wnt1 通过激活经典 Wnt 途径促进 SRA 表达。 Wnt1 通过与 SRA 结合增加 NF-kB 蛋白水平并增强 IL-6、TNF-α 和 iNOS 的分泌。这些发现表明 wnt1 增加了 SRA 和 NF-kB 蛋白水平并参与炎症反应。
The study investigated the role of wnt1 in the inflammatory response initiated by lipolysaccharide (LPS), and analyzed the association between wnt1, NF-KB, and inflammatory factors. THP-1 cells were activated with phorbol-12-myristate-13-acetate (PMA) and treated with LPS to induce inflammation. THP-1 cells were transfected with wnt1siRNA and overexpression plasmid to explore the relationship among wnt1, SRA, and NF-KB. Inhibitor of β-catenin and siRNA of FZD1were used to investigate the signaling events involved in SRA activation induced by wnt1. Levels of NF-kB protein and inflammatory cytokines were assessed followingwnt1 siRNA and LPS treatment. PMA activation and LPS treatment of THP-1 cells increased wnt1 protein levels. Wnt1 promoted SRA expression through activation of canonical wnt pathway. Wnt1 increased NF-kB protein levels and enhanced the secretion of IL-6, TNF-α, and iNOS through binding to SRA. These findings suggest that wnt1 increased SRA and NF-kB protein levels and participated in the inflammatory response.
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