Epigenetic regulation of sox30 is associated with testis development in mice.
Epigenetic regulation of sox30 is associated with testis development in mice.
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Sox30 的表观遗传调控与小鼠睾丸发育相关
DOI：
10.1371/journal.pone.0097203
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Han F;Dong Y;Liu W;Ma X;Shi R;Chen H;Cui Z;Ao L;Zhang H;Cao J;Liu J
DNA甲基化参与组织特异性和发育调控的基因表达。在此，我们筛选了一个新的甲基化基因Sox 30，其甲基化可能有助于其调控和小鼠睾丸发育。Sox 30是Sox转录因子家族的成员之一，被认为与精原细胞分化和精子发生有关。然而，确切的功能和调控表达模式仍不清楚。在本研究中，我们发现Sox 30在成年睾丸中高度表达，但在卵巢中不表达。Sox 30的表达开始于早期发育，在睾丸中，它特异性地随着发育而增加，直到成年。此外，Sox 30不仅在睾丸生殖细胞中表达，而且在支持细胞中也表达。Sox 30在成年小鼠的睾丸、附睾和肺中低甲基化，其中Sox 30表达。相比之下，Sox 30在成年小鼠的卵巢、心脏、脑、肝、肾、脾、胰腺、肌肉、肠、脑垂体、血液和海马中高甲基化，其中Sox 30不存在。重要的是，在出生后的小鼠发育睾丸中观察到Sox 30的CpG岛甲基化降低，这与Sox 30表达增强有关。然而，在此期间，Sox 30的高甲基化状态在不表达Sox 30的卵巢中维持。此外，在使用5-aza-dC进行去甲基化处理后，Sox 30表达在GC 2、TM 3和TM 4细胞系中恢复。这一观察结果令人信服地证实了甲基化确实有助于Sox 30沉默。总之，我们发现Sox 30的表达受DNA甲基化状态的控制，并且这种表达模式与小鼠睾丸发育相关。
DNA methylation is involved in tissue-specific and developmentally regulated gene expression. Here, we screened a novel methylation gene Sox30, whose methylation might contribute to its regulation and testis development in mice. Sox30 is a member of Sox transcription factors, and is considered to be involved in spermatogonial differentiation and spermatogenesis. However, the precise function and regulatory expression pattern remain unclear. In the present study, we found that Sox30 is highly expressed in adult testes but not in ovaries. Sox30 expression begins in early development, and in the testes, it is specifically increased coincidentally with development until adulthood. Moreover, Sox30 is expressed not only in testis germ cells, but also in sertoli cells. Sox30 is hypo-methylated in testis, epididymis and lung of adult mice, in which Sox30 is expressed. By contrast, Sox30 is hypermethylated in ovary, heart, brain, liver, kidney, spleen, pancreas, muscle, intestine, pituitary gland, blood and hippocampus of adult mice, in which the Sox30 is absent. Importantly, decreased methylation at CpG islands of Sox30 is observed in mouse developmental testes after birth, which is associated with enhanced Sox30 expression. However, the hypermethylated status of Sox30 is maintained in ovaries that does not express Sox30 during this period. Further, following demethylation treatment using 5-aza-dC, Sox30 expression is restored in GC2, TM3 and TM4 cell lines. This observation convincingly confirms that methylation really contributes to Sox30 silencing. In summary, we show that Sox30 expression is under the control of DNA methylation status, and this expression pattern is associated with testis development in mice.
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DOI：
10.1073/pnas.0508780102
发表时间：
2005-11-29
影响因子：
11.1
作者：
Hamra, FK;Chapman, KM;Garbers, DL
通讯作者：
Garbers, DL
影响因子：
64.5
作者：
Okano, M;Bell, DW;Li, E
通讯作者：
Li, E
影响因子：
2.6
作者：
Bernardino, J;Lombard, M;Dutrillaux, B
通讯作者：
Dutrillaux, B
影响因子：
4.8
作者：
Nicholls, Peter K.;Harrison, Craig A.;Stanton, Peter G.
通讯作者：
Stanton, Peter G.
影响因子：
3.6
作者：
Dirami, G;Ravindranath, N;Dym, M
通讯作者：
Dym, M