Proliferating dendritic cell progenitors in human blood.
Proliferating dendritic cell progenitors in human blood.
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DOI：
10.1084/jem.180.1.83
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发表时间：
1994-07-01
影响因子：
15.3
通讯作者：
Schuler, Gerold
中科院分区：
文献类型：
--
作者：
Romani, Nikolaus;Gruner, Stefan;Brang, Daniela;Kaempgen, Eckhart;Lenz, Angela;Trockenbacher, Bettina;Konwalinka, Guenther;Fritsch, Peter O.;Steinman, Ralph M.;Schuler, Gerold
已知人脐带血和骨髓中的CD 34+细胞产生树突状细胞（DC）以及其他骨髓谱系。然而，CD 34+细胞在成人血液中是罕见的，使得难以使用CD 34+细胞来确定DC祖细胞是否存在于循环中，以及血液是否可以作为获得大量这些免疫刺激性抗原呈递细胞用于临床研究的起点。因此，在几种情况下进行了对DC祖细胞的系统搜索。在每种情况下，我们最初寻找先前在小鼠中描述的独特的增殖聚集体。在脐血中，只需要去除红系祖细胞，并加入粒细胞/巨噬细胞集落刺激因子（GM-CSF）和肿瘤坏死因子（TNF），就可以观察到许多聚集体和典型DC子代的产生。在恶性肿瘤化疗后接受CSF的患者的成人血液中，GM-CSF和TNF同样从HLA-DR阴性前体产生特征性DC。然而，在来自健康供体的成人血液中，上述方法仅产生小的DC聚集体，其随后似乎变成单核细胞。当使用白细胞介素4抑制单核细胞发育时（Jansen，J.H.，G.- J. H. M.温特延斯，W. E.菲贝河Willemze和H. C. Kluin-Nelemans。1989. J. Exp. 170：577），GM-CSF的加入导致大的增殖性DC聚集体的形成，并且在5-7天内，导致许多非增殖性后代的形成，约3-8百万个细胞/40 ml血液。后代具有特征性的形态和表面组成（例如，丰富的HLA-DR和细胞介导免疫的辅助分子），并且是静止T细胞的有效刺激物。因此，大量的DC可以被来自血流中祖细胞的特异性细胞因子动员。这些相对大量的DC后代应该有助于未来对其Fc ε RI和CD 4受体的研究，以及它们在刺激T细胞介导的对病毒和肿瘤的抗性中的应用。
CD34+ cells in human cord blood and marrow are known to give rise to dendritic cells (DC), as well as to other myeloid lineages. CD34+ cells are rare in adult blood, however, making it difficult to use CD34+ cells to ascertain if DC progenitors are present in the circulation and if blood can be a starting point to obtain large numbers of these immunostimulatory antigen-presenting cells for clinical studies. A systematic search for DC progenitors was therefore carried out in several contexts. In each case, we looked initially for the distinctive proliferating aggregates that were described previously in mice. In cord blood, it was only necessary to deplete erythroid progenitors, and add granulocyte/macrophage colony-stimulating factor (GM-CSF) together with tumor necrosis factor (TNF), to observe many aggregates and the production of typical DC progeny. In adult blood from patients receiving CSFs after chemotherapy for malignancy, GM-CSF and TNF likewise generated characteristic DCs from HLA-DR negative precursors. However, in adult blood from healthy donors, the above approaches only generated small DC aggregates which then seemed to become monocytes. When interleukin 4 was used to suppress monocyte development (Jansen, J. H., G.-J. H. M. Wientjens, W. E. Fibbe, R. Willemze, and H. C. Kluin- Nelemans. 1989. J. Exp. Med. 170:577.), the addition of GM-CSF led to the formation of large proliferating DC aggregates and within 5-7 d, many nonproliferating progeny, about 3-8 million cells per 40 ml of blood. The progeny had a characteristic morphology and surface composition (e.g., abundant HLA-DR and accessory molecules for cell- mediated immunity) and were potent stimulators of quiescent T cells. Therefore, large numbers of DCs can be mobilized by specific cytokines from progenitors in the blood stream. These relatively large numbers of DC progeny should facilitate future studies of their Fc epsilon RI and CD4 receptors, and their use in stimulating T cell-mediated resistance to viruses and tumors.
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DOI：
10.1084/jem.153.6.1666
发表时间：
1981-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Barclay AN;Mayrhofer G
通讯作者：
Mayrhofer G
肿瘤坏死因子α维持鼠表皮兰格汉细胞在培养中的生存能力，但与粒细胞/巨噬细胞刺激因子相反，而没有诱导其功能成熟。
影响因子：
15.3
作者：
KOCH, F;HEUFLER, C;KAMPGEN, E;SCHNEEWEISS, D;BOCK, G;SCHULER, G
通讯作者：
SCHULER, G
白介素4对人类巨噬细胞形成的抑制4。
影响因子：
15.3
作者：
JANSEN, JH;WIENTJENS, GJHM;FIBBE, WE;WILLEMZE, R;KLUINNELEMANS, HC
通讯作者：
KLUINNELEMANS, HC
DOI：
10.1084/jem.177.5.1299
发表时间：
1993-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Liu LM;MacPherson GG
通讯作者：
MacPherson GG
DOI：
10.1073/pnas.90.7.3038
发表时间：
1993-04-01
影响因子：
11.1
作者：
INABA, K;INABA, M;STEINMAN, RM
通讯作者：
STEINMAN, RM