Upregulation of TLR2/4 expression in mononuclear cells in postoperative systemic inflammatory response syndrome after liver transplantation.
Upregulation of TLR2/4 expression in mononuclear cells in postoperative systemic inflammatory response syndrome after liver transplantation.
复制标题
肝移植术后全身炎症反应综合征中单核细胞TLR2/4表达上调
DOI：
10.1155/2010/519589
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发表时间：
2010
影响因子：
4.6
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Hei Z;Chi X;Cheng N;Luo G;Li S
背景资料。目的探讨肝移植术后患者外周血单个核细胞(PBMC)与全身炎症反应综合征(SIRS)的关系。方法：研究方法。分别于麻醉诱导后T1(麻醉诱导后)、T2(无肝期开始后25min)、T3(再灌流后3h)、T4(再灌流后24小时)采集27例肝移植患者的血样。检测外周血单核细胞表面TLR2/4的表达及血清肿瘤坏死因子-α、白介素1β和白介素8的浓度。将患者分为SIRS组(12例)和非SIRS组(15例)进行分析。结果。SIRS组的出血量和输血量均高于非SIRS组。SIRS组无肝前期和无肝期均明显延长。SIRS患者外周血单核细胞表面TLR2/4表达及血清肿瘤坏死因子-α、IL-1β、IL-8水平在T3、T4均显著高于T1、T2。外周血中TLR4的表达与血清肿瘤坏死因子-α、IL-8呈正相关。非SIRS患者外周血中TLR2/4的表达和血清中肿瘤坏死因子-α、IL-1β的浓度在4个时间点之间无明显差异。结论。PBMC表面TLR2/4表达上调可能参与了肝移植围手术期SIRS的发生。
Background. To explore the relationship between Toll-like rpheral blood mononuclear cells (PBMC) and systemic inflammatory response syndrome (SIRS) in postoperative patients of liver transplantation (LT). Methods. Blood samples of 27 patients receiving LT were collected at T1 (after induction of anaesthesia), T2 (25 minutes after the beginning of anhepatic phase), T3 (3 hours after graft reperfusion), and T4 (24 hours after graft reperfusion). The expression of TLR2/4 on PBMC and serum concentration of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-8 were measured. The patients were divided into SIRS group (n = 12) and non-SIRS group (n = 15) for analysis. Results. Blood loss and transfusion were higher in the SIRS group than in the non-SIRS group. Both the preanhepatic and anhepatic phase were significantly longer in the SIRS group. The TLR2/4 expression on PBMC as well as serum TNF-α, IL-1β, and IL-8 were significantly higher at T3 and T4 than that at T1 and T2 in the SIRS patients. The expression of TLR4 on PBMC is positively correlated to serum TNF-α, IL-8. Expression of TLR2/4 on PBMC and serum concentrations of TNF-α, IL-1β, did not differ among the 4-time points in non-SIRS patients. Conclusions. Upregulation of TLR2/4 expression on PBMC may contribute to the development of postoperative SIRS during perioperative period of LT.
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DOI：
10.1016/j.ejvs.2006.12.006
发表时间：
2007-06-01
影响因子：
5.7
作者：
Hassen, T. A.;Pearson, S.;Fitridge, R. A.
通讯作者：
Fitridge, R. A.
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作者：
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影响因子：
3.1
作者：
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通讯作者：
Rigato, Otelo
影响因子：
4.6
作者：
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Schmidt, Jan