Rapid exosomes concentration and in situ detection of exosomal microRNA on agarose-based microfluidic chip
Rapid exosomes concentration and in situ detection of exosomal microRNA on agarose-based microfluidic chip
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基于琼脂糖的微流控芯片上外泌体的快速浓缩和外泌体 microRNA 的原位检测
DOI：
10.1016/j.snb.2021.129559
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发表时间：
2021-04
影响因子：
8.4
通讯作者：
Liu Bi-Feng
中科院分区：
文献类型：
--
作者：
Qian Chungen;Xiao Yujin;Wang Jie;Li Yiwei;Li Shunji;Wei Bo;Du Wei;Feng Xiaojun;Chen Peng;Liu Bi-Feng
外泌体及其伴随的蛋白质和核酸已越来越多地被认为是非侵入性肿瘤诊断和预后的生物标志物。然而，由于外泌体的小尺寸（30 - 150 nm）和低浓度，快速有效地检测外泌体仍然具有挑战性。因此，为了保证，特别是当癌症筛查需要检测限低于外泌体浓度时，它不仅需要分离和纯化外泌体，而且还需要预浓缩它们。本研究开发了一种低成本、快速、便携的基于琼脂糖的微流控芯片，用于exosome的浓缩和exosomal microRNA的原位检测，称为isExoCD（in situ exosome concentration and detection）。通过利用毛细管效应和琼脂糖凝胶的强透水性，在入口处加载的靶外泌体将在微通道的封闭端自动富集。为了检测外泌体microRNA，我们将基于催化发夹组装（CHA）的策略整合到isExoCD中，从而允许高灵敏度检测外泌体，检测限（LOD）为1003。使用我们的方法，我们可以成功地将癌细胞来源的外泌体与获得其他细胞类型的其他外泌体区分开来。因此，我们的平台为早期癌症检测、液体活检和即时诊断铺平了新的道路。
Exosomes, together with their accompanying proteins and nucleic acids have been increasingly recognized as biomarkers for non-invasive tumor diagnostic and prognostic. However, rapid and efficient detection of exosomes remains challenging, due to their small size (30−150 nm), and low concentration. Thus, to guarantee, especially when cancer screening requires detection limits lower than exosome concentrations, it not only needs to isolate and purify exosomes, but it needs to preconcentrate them as well. Here, a low-cost, rapid, and portable agarose-based microfluidic chip for exosome concentration andin situexosomal microRNA detection was developed, termed isExoCD (in situ exosome concentration and detection). The target exosomes loaded at the entrance will be automatically enriched at the closed end of the microchannel by leveraging the capillary effect and the strong water permeability of the agarose gel. To detect the exosomal microRNA, we integrated the catalyzed hairpin assembly (CHA) based strategy into the isExoCD, allowing high sensitive detection of exosomes with a limit of detection (LOD) of ∼103. Using our method, we can successfully distinguish cancer cell-derived exosomes from other exosomes that obtained other cell types. Thus, our platform paves a new avenue to early cancer detection, liquid biopsy, and point-of-care diagnosis.
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影响因子：
2.9
作者：
Marczak S;Richards K;Ramshani Z;Smith E;Senapati S;Hill R;Go DB;Chang HC
通讯作者：
Chang HC
DOI：
10.1002/anie.201806901
发表时间：
2018-11-26
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Shen W;Guo K;Adkins GB;Jiang Q;Liu Y;Sedano S;Duan Y;Yan W;Wang SE;Bergersen K;Worth D;Wilson EH;Zhong W
通讯作者：
Zhong W
影响因子：
10.8
作者：
Rodrigues M;Richards N;Ning B;Lyon CJ;Hu TY
通讯作者：
Hu TY
影响因子：
13.3
作者：
Jokerst, Jesse V.;Chou, Jie;Camp, James P.;Wong, Jorge;Lennart, Alexis;Pollard, Amanda A.;Floriano, Pierre N.;Christodoulides, Nicolaos;Simmons, Glennon W.;Zhou, Yanjie;Ali, Mehnaaz F.;McDevitt, John T.
通讯作者：
McDevitt, John T.
影响因子：
17.1
作者：
Woo, Hyun-Kyung;Sunkara, Vijaya;Cho, Yoon-Kyoung
通讯作者：
Cho, Yoon-Kyoung