Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip.
Location of biomarkers and reagents within agarose beads of a programmable bio-nano-chip.
复制标题
DOI：
10.1002/smll.201002089
复制
发表时间：
2011-03-07
期刊：
影响因子：
13.3
通讯作者：
McDevitt, John T.
中科院分区：
文献类型：
--
作者：
Jokerst, Jesse V.;Chou, Jie;Camp, James P.;Wong, Jorge;Lennart, Alexis;Pollard, Amanda A.;Floriano, Pierre N.;Christodoulides, Nicolaos;Simmons, Glennon W.;Zhou, Yanjie;Ali, Mehnaaz F.;McDevitt, John T.
由于缺乏选择性和高效的分析物捕获和信号，具有强大分析性能特征的经济高效医疗微设备的发展缓慢。最近开发的可编程生物纳米芯片（PBNC）是一种灵活的检测设备，其分析行为可与现有的宏观方法相媲美。PBNC系统采用直径约为300 μm的磁珠传感器，由琼脂糖“纳米”组成，填充在集成微流控元件的微机支撑结构中。珠子是一种高效和选择性的蛋白质捕获介质，适用于复杂流体样品的分析。显微镜和计算机研究探测珠子的三维内部。报告了捕获和检测的组分、分析物的大小和颗粒孔隙度对信号分布和强度的相对贡献。琼脂糖的孔径范围为45 ~ 620 nm，接近140 nm的孔径为快速（<15分钟）测试提供了最佳的传输特性。该系统具有高效（99.5%）的检测珠结合分析物，以及低（≈2%）的非特异性固定检测探针用于癌胚抗原测定。此外，分析物尺寸在信号分布中所起的作用进行了探索，并提供了考虑生物标志物尺寸独特特征的分析方法。
The slow development of cost-effective medical microdevices with strong analytical performance characteristics is due to a lack of selective and efficient analyte capture and signaling. The recently developed programmable bio-nano-chip (PBNC) is a flexible detection device with analytical behavior rivaling established macroscopic methods. The PBNC system employs ≈300 μm-diameter bead sensors composed of agarose “nanonets” that populate a microelectromechanical support structure with integrated microfluidic elements. The beads are an efficient and selective protein-capture medium suitable for the analysis of complex fluid samples. Microscopy and computational studies probe the 3D interior of the beads. The relative contributions that the capture and detection of moieties, analyte size, and bead porosity make to signal distribution and intensity are reported. Agarose pore sizes ranging from 45 to 620 nm are examined and those near 140 nm provide optimal transport characteristics for rapid (<15 min) tests. The system exhibits efficient (99.5%) detection of bead-bound analyte along with low (≈2%) nonspecific immobilization of the detection probe for carcinoembryonic antigen assay. Furthermore, the role analyte dimensions play in signal distribution is explored, and enhanced methods for assay building that consider the unique features of biomarker size are offered.
登录
查看更多内容
影响因子：
5.6
作者：
Harris, LJ;Skaletsky, E;McPherson, A
通讯作者：
McPherson, A
影响因子：
7.4
作者：
Borowsky, Joseph F.;Giordano, Braden C.;Collins, Greg E.
通讯作者：
Collins, Greg E.
影响因子：
7.4
作者：
Gertsch, Jana C.;Noblitt, Scott D.;Henry, Charles S.
通讯作者：
Henry, Charles S.
影响因子：
4.2
作者：
Grate, Jay W.;Warner, Marvin G.;Bruckner-Lea, Cynthia J.
通讯作者：
Bruckner-Lea, Cynthia J.
影响因子：
3.7
作者：
Chang, Hsueh-Chia
通讯作者：
Chang, Hsueh-Chia