Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis.
Assessing a novel room-temperature RNA storage medium for compatibility in microarray gene expression analysis.
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DOI：
10.2144/000113209
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发表时间：
2009-08
期刊：
影响因子：
2.7
通讯作者：
Head SR
中科院分区：
文献类型：
--
作者：
Hernandez GE;Mondala TS;Head SR
RNA完整性是获得有意义的基因表达数据的关键因素。目前的方法依赖于在采集、运输、处理和储存过程中在寒冷的环境中保存样品，这些程序也对时间非常敏感。市面上有几种RNA储存产品可帮助防止在搬运和储存步骤中降解；然而，样品必须冷藏以获得最佳保护。我们评估了一种基于无水生物作用的新型RNA存储介质，用于稳定和防止样品在室温下降解，用于微阵列分析。样品在室温下干燥储存不同的时间段，以评估与冷冻对照样品相比是否有任何降解或活性损失。回收的样品直接用于分析，不需要进一步纯化，在下游应用中没有显示出干扰或抑制。基因表达谱的比较表明，对照冷藏样品和室温保存在RNA存储介质中的样品没有显著差异。用分光光度法和生物分析仪对回收的RNA进行了鉴定，结果与对照样品一致。在环境温度下长时间稳定RNA样品的能力将具有巨大的用途，特别是在将样品运送到核心设施的过程中。
RNA integrity is a critical factor in obtaining meaningful gene expression data. Current methodologies rely on maintaining samples in cold environments during the collection, transport, processing and storage procedures that are also extremely time-sensitive. Several RNA storage products are commercially available to help prevent degradation during the handling and storage steps; however, samples must be kept cold for optimal protection. We have evaluated a novel RNA storage medium based on anhydrobiosis for stabilizing and protecting samples from degradation at room temperature that are intended for use in microarray analysis. Samples were stored dry at room temperature for various time periods to assess any degradation or loss of activity as compared to frozen control samples. Recovered samples were used directly for analysis without further purification and exhibited no interference or inhibition in downstream applications. Comparison of gene expression profiles indicate no significant differences between control freezer stored samples and those kept at room temperature protected in the RNA storage medium. The quality of recovered RNA was confirmed using spectrophotometry and bioanalyzer analysis and was identical to control samples. The ability to stabilize RNA samples at ambient temperatures for extended time periods will have tremendous use, particularly for sample shipment to core facilities.
DOI：
10.1016/s0076-6879(06)16010-3
发表时间：
2006-01-01
期刊：
GLYCOMICS
影响因子：
--
作者：
Hammond, Jennifer A.;Head, Steven R.
通讯作者：
Head, Steven R.
影响因子：
4.4
作者：
Mutter GL;Zahrieh D;Liu C;Neuberg D;Finkelstein D;Baker HE;Warrington JA
通讯作者：
Warrington JA
影响因子：
2
作者：
Kasahara, Toshihiko;Miyazaki, Toshiko;Urushidani, Tetsuro
通讯作者：
Urushidani, Tetsuro