The yeast global transcriptional co-repressor protein Cyc8 can propagate as a prion.
The yeast global transcriptional co-repressor protein Cyc8 can propagate as a prion.
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DOI：
10.1038/ncb1843
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发表时间：
2009-03
影响因子：
21.3
通讯作者：
Liebman, Susan W.
中科院分区：
文献类型：
--
作者：
Patel, Basant K.;Gavin-Smyth, Jackie;Liebman, Susan W.
虽然许多蛋白质可以错误折叠成自播种的类淀粉样构象，但只有6种蛋白质是已知的具有感染性的，即：[PSI+]、[PIN+]、[URE3]、[SWI+]和[HET-S]在真菌中引起明显的可遗传的生理变化，而PrPSc在哺乳动物中引起感染性脑病。目前尚不清楚“纯蛋白质”遗传是否仅限于这些特殊情况，或者代表了一种广泛的表观遗传控制机制。为了实现这一目标，我们现在描述了一种由酿酒酵母Cyc8(Ssn6)蛋白形成的新的Prion。与其他酵母蛋白类似，Cyc8谷氨酰胺富集区的瞬时过量生产诱导了一种可遗传的显性Cyc8−表型，该表型通过细胞质传递，依赖于伴侣热休克蛋白104和Cyc8蛋白的持续存在。在进化上保守的Cyc8-Tup1全球转录抑制因子复合体形成了最大的基因调控电路之一，控制着超过7%的酵母基因的表达。我们的发现是Cyc8可以作为Prion进行繁殖，再加上最近的一篇报道，即Swi-SNF全球转录调控复合体的Swi1也具有Prion形式，表明Pri作用可以导致酵母基因的大量激活或抑制，并提示染色质重塑和Prion形成的表观遗传现象之间存在联系。
Although many proteins can misfold into a self-seeding amyloid-like conformation, only six are known to be infectious, i.e. prions; [PSI+], [PIN+], [URE3], [SWI+] and [HET-s] cause distinct heritable physiological changes in fungi, while PrPSc causes infectious encephalopathies in mammals. It is unknown if “protein-only” inheritance is limited to these exceptional cases, or represents a widespread mechanism of epigenetic control. Towards this goal, we now describe a new prion formed by the Cyc8 (Ssn6) protein of Saccharomyces cerevisiae. Analogous to other yeast prions, transient over-production of a glutamine-rich region of Cyc8 induced a heritable dominant cyc8− phenotype that is transmitted cytoplasmically and dependent on the chaperone Hsp104 and the continued presence of the Cyc8 protein. The evolutionarily conserved Cyc8-Tup1 global transcriptional repressor complex forms one of the largest gene regulatory circuits, controlling the expression of over 7% of yeast genes. Our finding that Cyc8 can propagate as a prion, together with a recent report that Swi1 of the Swi-Snf global transcriptional regulatory complex also has a prion form, shows that prionization can lead to mass activation or repression of yeast genes and is suggestive of a link between the epigenetic phenomena of chromatin remodeling and prion formation.
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影响因子：
3.2
作者：
MIKI, BLA;POON, NH;SELIGY, VL
通讯作者：
SELIGY, VL
新发现的朊病毒与酿酒酵母中染色质重塑因子 Swi1 相关
影响因子：
30.8
作者：
Du, Zhiqiang;Park, Kyung-Won;Li, Liming
通讯作者：
Li, Liming
影响因子：
5.3
作者：
SCHULTZ, J;MARSHALLCARLSON, L;CARLSON, M
通讯作者：
CARLSON, M
影响因子：
5.6
作者：
Patel, Basant K.;Liebman, Susan W.
通讯作者：
Liebman, Susan W.
影响因子：
56.9
作者：
Patino, MM;Liu, JJ;Lindquist, S
通讯作者：
Lindquist, S