Sparstolonin B suppresses lipopolysaccharide-induced inflammation in human umbilical vein endothelial cells.
Sparstolonin B suppresses lipopolysaccharide-induced inflammation in human umbilical vein endothelial cells.
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Sparstolonin B 抑制脂多糖诱导的人脐静脉内皮细胞炎症
DOI：
10.1007/s12272-013-0120-8
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发表时间：
2013-07
影响因子：
6.7
通讯作者：
Fan, Daping
中科院分区：
文献类型：
--
作者：
Liang, Qiaoli;Yu, Fang;Cui, Xiaodong;Duan, Jin'ao;Wu, Qinan;Nagarkatti, Prakash;Fan, Daping
玄参甲素B(SsnB)是从三棱和三棱中分离得到的一种异香豆素类化合物。我们先前证明SSNB通过抑制MyD88募集到TLR2和TLR4的TIR结构域来阻断Toll样受体(TLR)2和TLR4触发的巨噬细胞炎症信号转导。本研究旨在探讨丹参B对脂多糖(TLR4配体)刺激的人脐静脉内皮细胞(HUVECs)血管炎症反应的影响。我们发现丹参B在转录和翻译水平上均呈剂量依赖性地抑制脂多糖诱导的人脐静脉内皮细胞IL-1β和单核细胞趋化蛋白1的表达。丹参B还能降低脂多糖诱导的内皮细胞黏附分子、细胞间黏附分子-1和血管细胞黏附分子-1的表达。此外，与SSNB共同孵育可降低THP-1单核细胞与脂多糖激活的HUVECs的黏附。此外，SSNB还能有效抑制内毒素诱导的人脐静脉内皮细胞胞外信号调节激酶(ERK1/2)和Akt的磷酸化。这些发现表明SSNB具有抑制内皮细胞炎症的作用，提示SSNB可能适合作为炎症性心血管疾病的治疗剂开发。
Sparstolonin B (SsnB) is an isocoumarin compound isolated from the tubers of bothSparganium stoloniferumandScirpus yagara. We previously demonstrated that SsnB blocked the Toll-like receptor (TLR) 2- and TLR4-triggered inflammatory signaling in macrophages by inhibiting the recruitment of MyD88 to the TIR domains of TLR2 and TLR4. The present study was designed to examine the effects of SsnB on vascular inflammatory responses in human umbilical vein endothelial cells (HUVECs) challenged by lipopolysaccharide (LPS, a TLR4 ligand). We found that SsnB dose-dependently attenuated the LPS-induced expression of interleukin (IL)-1β and monocyte chemoattractant protein 1 both at the transcription and translation levels in HUVEC. LPS-induced endothelial cell adhesion molecules, intercellular adhesion molecular-1 and vascular cell adhesion molecule-1 expressions were also reduced by treatment with SsnB. In addition, co-incubation with SsnB attenuated THP-1 monocyte adhesion to LPS-activated HUVECs. Furthermore, SsnB efficiently suppressed LPS-induced phosphorylation of extracellular -signal-regulated kinase (Erk1/2) and Akt in HUVECs. These findings show that SsnB can suppress endothelial cell inflammation, suggesting that SsnB might be suitable for development as a therapeutic agent for inflammatory cardiovascular disease.
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影响因子：
6.7
作者：
Liu, Hong-Tao;He, Jun-Lin;Yu, Chao
通讯作者：
Yu, Chao
动脉粥样硬化的炎症。
DOI：
10.1161/atvbaha.108.179705
发表时间：
2012-09
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Libby P
通讯作者：
Libby P
影响因子：
5.4
作者：
Moon, Lidiya;Ha, Yu Mi;Chang, Ki Churl
通讯作者：
Chang, Ki Churl
DOI：
10.1161/atvbaha.109.196360
发表时间：
2010-02-01
影响因子：
8.7
作者：
Bains, Sandip K.;Foresti, Roberta;Motterlini, Roberto
通讯作者：
Motterlini, Roberto
影响因子：
6.2
作者：
Dengler, TJ;Raftery, MJ;Schönrich, G
通讯作者：
Schönrich, G