Salvianolic acid-B improves fat graft survival by promoting proliferation and adipogenesis.
Salvianolic acid-B improves fat graft survival by promoting proliferation and adipogenesis.
复制标题
丹酚酸-B 通过促进增殖和脂肪生成来提高脂肪移植物的存活率
DOI：
10.1186/s13287-021-02575-4
复制
发表时间：
2021-09-17
影响因子：
7.5
通讯作者：
Yu L
中科院分区：
文献类型：
--
作者：
Sun JM;Ho CK;Gao Y;Chong CH;Zheng DN;Zhang YF;Yu L
背景我们前期的研究证明丹参可以通过促进脂肪生成来提高脂肪移植物的存活率。然而，丹参中含量最丰富、生物活性最强的水溶性化合物丹酚酸B（Sal-B）对脂肪移植物存活的影响尚未被研究。目的本研究旨在探讨丹酚酸B是否可以提高脂肪移植物的存活率并促进前脂肪细胞分化。方法在体内，将 0.2 ml Coleman 脂肪移植到含有丹酚酸 B 的裸鼠体内。移植后 2 周和 4 周通过 HE 和 IF 评估移植物，并在移植后 4 周通过微型 CT 评估移植物。体外培养的人脂肪干细胞（h-ADSC）和3T3-L1细胞中，分析丹酚酸B的脂肪形成和增殖活性，以探讨丹酚酸B影响移植物存活的机制。结果在体内，丹酚酸B处理组的脂肪移植物的重量和体积均显着高于对照组的脂肪移植物。此外，在 Sal-B 处理组中观察到更高的脂肪完整性和更多活性脂肪细胞。在体外，丹酚酸B表现出促进3T3-L1和h-ADSC增殖和脂肪形成的能力。结论我们的体外实验表明丹酚酸B可以促进脂肪干细胞的增殖并增强脂肪干细胞的分化。同时，体内实验表明丹酚酸B可以提高脂肪移植的成活率。因此，我们的研究揭示了丹酚酸 B 在提高脂肪移植成活率方面的潜在治疗用途。
BackgroundOur previous study proved thatSalvia miltiorrhizacould enhance fat graft survival by promoting adipogenesis. However, the effect of salvianolic acid B (Sal-B), the most abundant and bioactive water-soluble compound inSalvia miltiorrhiza, on fat graft survival has not yet been investigated.ObjectiveThis study aims to investigate whether salvianolic acid B could improve fat graft survival and promote preadipocyte differentiation. The underlying mechanism has also been studied.MethodsIn vivo, 0.2 ml of Coleman fat was transplanted into nude mice with salvianolic acid B. The grafts were evaluated by HE and IF at 2 and 4 weeks posttransplantation and by micro-CT at 4 weeks posttransplantation. In vitro, the adipogenesis and proliferative activities of salvianolic acid B were analyzed in cultured human adipose-derived stem cells (h-ADSCs) and 3T3-L1 cells to detect the mechanism by which salvianolic acid B affects graft survival.ResultsIn vivo, the weights and volumes of the fat grafts in the Sal-B-treated groups were significantly higher than those of the fat grafts in the control group. In addition, higher fat integrity and more viable adipocytes were observed in the Sal-B-treated groups. In vitro, salvianolic acid B showed the ability to promote 3T3-L1 and h-ADSC proliferation and adipogenesis.ConclusionsOur in vitro experiments demonstrated that salvianolic acid B can promote the proliferation of adipose stem cells and enhance the differentiation of adipose stem cells. Simultaneously, in vivo experiments showed that salvianolic acid B can improve the survival rate of fat transplantation. Therefore, our research shed light on the potential therapeutic usage of salvianolic acid B in improving the survival rate of fat transplantation.
登录
查看更多内容
低剂量 G-CSF 通过动员内源干细胞和诱导血管生成来改善脂肪移植保留，而高剂量 G-CSF 会抑制脂肪生成，导致长期炎症和严重纤维化
DOI：
10.1016/j.bbrc.2017.07.147
发表时间：
2017-09-23
影响因子：
3.1
作者：
Cai, Junrong;Li, Bin;Lu, Feng
通讯作者：
Lu, Feng
使用转染 VEGF 的脂肪干细胞提高人类自体脂肪移植的存活率
影响因子：
3.6
作者：
Lu, Feng;Li, Jie;Fu, Bingchuan
通讯作者：
Fu, Bingchuan
影响因子：
3.6
作者：
Ruan, Qing Zhao;Rinkinen, Jacob R.;Lee, Bernard T.
通讯作者：
Lee, Bernard T.
影响因子：
3.6
作者：
Hong, Ki Yong;Yim, Sangjun;Minn, Kyung Won
通讯作者：
Minn, Kyung Won
影响因子：
29
作者：
Liu, J;DeYoung, SM;Saltiel, AR
通讯作者：
Saltiel, AR