Temporal and spatial distribution of gentamicin in the peripheral vestibular system after transtympanic administration in guinea pigs.
Temporal and spatial distribution of gentamicin in the peripheral vestibular system after transtympanic administration in guinea pigs.
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豚鼠经鼓膜给药后庆大霉素在外周前庭系统中的时空分布
DOI：
10.1016/j.heares.2013.01.010
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发表时间：
2013-04
期刊：
影响因子：
2.8
通讯作者：
Steyger PS
中科院分区：
文献类型：
--
作者：
Zhang R;Zhang YB;Dai CF;Steyger PS
庆大霉素的胞胰蛋白酶给药可有效治疗棘手的眩晕患者。这项研究探讨了胞质剂给药后前庭末端源中庆大霉素的空间和时间分布。三十几只豚鼠被偶然注射到庆大霉素与德克萨斯红色（GTTR）及其前庭末端，后者在各种生存期后检查。另外9只豚鼠以不同剂量接受GTTR。九只动物仅接受德克萨斯州的红色，并用作对照。我们使用共聚焦显微镜来确定GTTR在半圆形管的cristae，以及尿布和腹膜的细胞分布。与其他前庭端孔相比，在肺泡中存在最强烈的GTTR标记。在单次三型毛细胞，II型毛细胞和过渡细胞中主要检测到GTTR荧光，在单次跨性剂量的GTTR（0.1 mg/ml，0.05 mL）之后，仅观察到，而仅在非感官细胞中观察到弱荧光，例如支持细胞，暗细胞，黑暗细胞，黑暗细胞和液体上皮细胞。过渡细胞在跨抑制注射后24小时表现出强烈的GTTR荧光，至少保留了4周。观察到GTTR荧光的空间梯度降低，其中含有I型I型I型I型I型的感觉上皮区域，然后在Crista Ampullaris中的II型毛细胞，从striolar到前毛的毛细胞中的II型毛细胞。 GTTR荧光从限制到较低剂量的顶端细胞质延伸到剂量增加的整个I型毛细胞的整个细胞体。 GTTR荧光达到了骨膜注射后3至7天之间在Cristae和Makulae中各个感兴趣区域的峰值强度。在半圆形管中，GTTR的晶状体摄取比其他前庭端孔大。
Transtympanic administration of gentamicin is effective for treating patients with intractable vertigo. This study explored the spatial and temporal distribution of gentamicin in vestibular end-organs after transtympanic administration. Thirty guinea pigs were transtympanically injected with gentamicin conjugated to Texas Red (GTTR) and their vestibular end-organs examined after various survival periods. Another 9 guinea pigs received GTTR at different doses. Nine animals received Texas Red only and served as controls. We used confocal microscopy to determine the cellular distribution of GTTR in semicircular canal cristae, as well as the utricular and saccular maculae. The most intense GTTR labeling was present in the saccule compared to other vestibular end-organs. GTTR fluorescence was detected predominantly in type I hair cells, type II hair cells and transitional cells after a single transtympanic dose of GTTR (0.1 mg/ml, 0.05 ml), while only weak fluorescence was observed in non-sensory cells such as supporting cells, dark cells and lumenal epithelial cells. Transitional cells displayed intense GTTR fluorescence in the supra-nuclear regions 24 h after transtympanic injection that was retained for at least 4 weeks. A decreasing spatial gradient of GTTR fluorescence was observed sensory epithelial regions containing central type I to peripheral type I and then type II hair cells in the crista ampullaris, and from striolar to extra-striolar hair cells within the vestibular macula. GTTR fluorescence extended from being restricted to the apical cytoplasm at lower doses to the entire cell body of type I hair cells with increasing dose. GTTR fluorescence reached peak intensities for individual regions of interest within the cristae and maculae between 3 and 7 days after transtympanic injection. The saccular uptake of GTTR is greater than other vestibular end-organs after transtympanic injection in the semicircular canals.
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影响因子：
3.8
作者：
Karasawa, Takatoshi;Wang, Qi;Steyger, Peter S.
通讯作者：
Steyger, Peter S.
影响因子：
2.5
作者：
Hirvonen, TP;Minor, LB;Carey, JP
通讯作者：
Carey, JP
影响因子：
1.4
作者：
LINDEMAN, HH
通讯作者：
LINDEMAN, HH
影响因子：
2.9
作者：
Hashino, E;Shero, M
通讯作者：
Shero, M
DOI：
10.1007/s10162-002-2036-8
发表时间：
2003-06-01
影响因子：
2.4
作者：
Imamura, S;Adams, JC
通讯作者：
Adams, JC