Peanuts can contribute to anaphylactic shock by activating complement.
Peanuts can contribute to anaphylactic shock by activating complement.
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DOI：
10.1016/j.jaci.2008.11.004
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发表时间：
2009-02
影响因子：
14.2
通讯作者：
Finkelman, Fred D.
中科院分区：
文献类型：
--
作者：
Khodoun, Marat;Strait, Richard;Orekov, Tatyana;Hogan, Simon;Karasuyama, Hajime;Herbert, De'Broski R.;Koehl, Joerg;Finkelman, Fred D.
关键词：
花生过敏是与食物有关的致命性过敏反应最常见的原因，与其他食物过敏不同，花生过敏通常会持续到成年。花生的主要过敏原Ara H1对消化的抵抗和树突状细胞的激活被报道与其过敏性有关。评估花生分子是否也可能通过先天免疫机制促进过敏反应。用β肾上腺素能受体拮抗剂和长效IL-4治疗幼稚小鼠以增加对血管活性物质的敏感性，并注射花生提取物(PE)。用直肠测温仪检测休克情况，并进行量化。基因缺陷小鼠和特异性拮抗剂被用来确定特定细胞类型、补体、Fc受体和血管活性介质在休克发病机制中的作用。1)PE诱导剂量依赖性休克；2)PE在小鼠体内和体外激活补体；3)C3a，刺激免疫球蛋白(Ig)受体在PE休克中的作用较小；4)PE休克更多地依赖巨噬细胞和嗜碱性粒细胞而不是肥大细胞；5)血小板活化因子和较小程度的组胺参与PE休克；6)PE在缺乏适应性免疫系统的情况下诱导休克；7)内毒素污染不是PE休克的原因；8)PE和IgE介导的肥大细胞脱颗粒协同诱导休克；9)坚果和PE有相似的作用，脱脂牛奶和蛋清则不然。花生可通过产生C3a刺激巨噬细胞、嗜碱性粒细胞和肥大细胞产生PAF和组胺，从而导致休克。
Peanut allergy is the most common food-related cause of lethal anaphylaxis and, unlike other food allergies, typically persists into adulthood. Resistance to digestion and dendritic cell activation by the major peanut allergen, Ara h1, are reported to contribute to its allergenicity. Evaluate whether peanut molecules may also promote anaphylaxis through an innate immune mechanism. Naïve mice were treated with a β-adrenergic receptor antagonist and long-acting IL-4 to increase sensitivity to vasoactive mediators and injected with peanut extract (PE). Shock was detected and quantified by rectal thermometry. Gene-deficient mice and specific antagonists were used to determine the roles of specific cell types, complement, Fc receptors, and vasoactive mediators in shock pathogenesis. 1) PE induces dose-dependent shock; 2) PE activates complement in vivo in mice and in vitro in mice and humans; 3) C3a, and, to a lesser extent, stimulatory immunoglobulin (Ig) receptors contribute to PE-induced shock; 4) PE-induced shock depends more on macrophages and basophils than on mast cells; 5) platelet activating factor and, to a lesser extent, histamine contribute to PE-induced shock; 6) PE induces shock in the absence of the adaptive immune system; 7) LPS contamination is not responsible for PE-induced shock; 8) PE and IgE-mediated mast cell degranulation synergistically induce shock; and 9) Tree nuts have similar effects to PE; skim milk and egg white do not. Peanuts can contribute to shock by causing production of C3a, which stimulates macrophages, basophils and mast cells to produce PAF and histamine.
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影响因子：
5.4
作者：
CAVAILLON, JM;FITTING, C;HAEFFNERCAVAILLON, N
通讯作者：
HAEFFNERCAVAILLON, N
影响因子：
14.2
作者：
Bock, SA;Muñoz-Furlong, A;Sampson, HA
通讯作者：
Sampson, HA
影响因子：
4.4
作者：
Kojima, Toshiyuki;Obata, Kazushige;Karasuyama, Hajime
通讯作者：
Karasuyama, Hajime
影响因子：
7.3
作者：
BECKER, EL;MOTA, I;WONG, D
通讯作者：
WONG, D
影响因子：
4.4
作者：
Kildsgaard, J;Hollmann, TJ;Wetsel, RA
通讯作者：
Wetsel, RA