Shape shifter: redirection of prolate phage capsid assembly by staphylococcal pathogenicity islands.
Shape shifter: redirection of prolate phage capsid assembly by staphylococcal pathogenicity islands.
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DOI：
10.1038/s41467-021-26759-x
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发表时间：
2021-11-04
影响因子：
16.6
通讯作者：
Dokland T
中科院分区：
文献类型：
--
作者：
Hawkins NC;Kizziah JL;Penadés JR;Dokland T
Staphylococcus aureus pathogenicity islands (SaPIs) are molecular parasites that hijack helper phages for their transfer. SaPIbov5, the prototypical member of a family of cos type SaPIs, redirects the assembly of ϕ12 helper capsids from prolate to isometric. This size and shape shift is dependent on the SaPIbov5-encoded protein Ccm, a homolog of the ϕ12 capsid protein (CP). Using cryo-electron microscopy, we have determined structures of prolate ϕ12 procapsids and isometric SaPIbov5 procapsids. ϕ12 procapsids have icosahedral end caps with Tend = 4 architecture and a Tmid = 14 cylindrical midsection, whereas SaPIbov5 procapsids have T = 4 icosahedral architecture. We built atomic models for CP and Ccm, and show that Ccm occupies the pentameric capsomers in the isometric SaPIbov5 procapsids, suggesting that preferential incorporation of Ccm pentamers prevents the cylindrical midsection from forming. Our results highlight that pirate elements have evolved diverse mechanisms to suppress phage multiplication, including the acquisition of phage capsid protein homologs. Phage-inducible chromosomal islands (PICIs) are a group of mobile genetic elements that hijack the replication and assembly machinery of helper bacteriophages. Here the authors describe a mechanism by which a group of PICIs from Staphylococcus aureus re-direct the assembly pathway of their helpers using a capsid protein homolog.
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DOI：
10.1038/ismej.2016.163
发表时间：
2017-04
期刊：
The ISME journal
影响因子：
--
作者：
Martínez-Rubio R;Quiles-Puchalt N;Martí M;Humphrey S;Ram G;Smyth D;Chen J;Novick RP;Penadés JR
通讯作者：
Penadés JR
影响因子：
3.4
作者：
CASPAR, DLD
通讯作者：
CASPAR, DLD
影响因子：
3.7
作者：
NOVICK, R
通讯作者：
NOVICK, R
影响因子：
6.7
作者：
Kizziah, James L.;Manning, Keith A.;Dokland, Terje
通讯作者：
Dokland, Terje
DOI：
10.1107/s2059798318006551
发表时间：
2018-06-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Afonine PV;Poon BK;Read RJ;Sobolev OV;Terwilliger TC;Urzhumtsev A;Adams PD
通讯作者：
Adams PD