Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA.

Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA.
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DOI:
10.1038/nchembio.2228
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发表时间:
2017-01
影响因子:
14.8
通讯作者:
Dominguez C
Dominguez C
中科院分区:
生物学1区
文献类型:
--
作者:
Weldon C;Behm-Ansmant I;Hurley LH;Burley GA;Branlant C;Eperon IC;Dominguez C

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RNA G-四链体(G4)结构被认为影响生物学过程,包括翻译和前mRNA剪接,但目前还不可能证明它们在长功能RNA分子内的特定序列上天然形成。我们已经开发了一种新的策略,长7-脱氮鸟嘌呤取代的RNA(FOLDeR)的足迹,它允许在长RNA和功能条件下确认G4的形成。
RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences within long functional RNA molecules. We have developed a novel strategy, footprinting of long 7-deazaguanine substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and in functional conditions.
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