Physical map of the Treponema denticola circular chromosome
Physical map of the Treponema denticola circular chromosome
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密螺旋体环形染色体物理图
DOI：
10.1128/jb.177.7.1805-1811.1995
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发表时间：
1995
影响因子：
3.2
通讯作者：
I. Saint Girons
中科院分区：
文献类型：
--
作者：
J. Macdougall;I. Saint Girons
通过脉冲场凝胶电泳和DNA杂交构建齿垢密螺旋体ATCC 33520基因组的物理图谱。该生物体具有约3.0 Mbp的单一环状染色体和2.6 kbp的环状质粒pTD 1。富含A+ T基因组的物理图谱是用稀有切割限制性内切酶AscI、NotI和SrfI构建的，这些酶具有8 bp富含G+ C的识别位点。基因flgE，tdpA和prtB编码的鞭毛钩蛋白，53 kDa的免疫原性蛋白，胰凝乳蛋白酶样蛋白酶，分别位于地图上。发现该密螺旋体具有每个rRNA基因的两个拷贝，如已经发现的噬菌体密螺旋体和苍白密螺旋体的情况。
A physical map of the Treponema denticola ATCC 33520 genome was constructed by pulsed-field gel electrophoresis and DNA hybridization. The organism possesses a single, circular chromosome of approximately 3.0 Mbp and a 2.6-kbp circular plasmid, pTD1. The physical map of the A+T-rich genome was constructed with the rare-cutting restriction enzymes AscI, NotI, and SrfI, which have 8-bp G+C-rich recognition sites. The genes flgE, tdpA, and prtB encoding the flagellar hook protein, a 53-kDa immunogenic protein, and chymotrypsinlike protease, respectively, were located on the map. This treponeme was found to have two copies of each of the rRNA genes, as has been found to be the case for both Treponema phagedenis and Treponema pallidum.
人类中性粒细胞与斑块螺旋体相互作用的研究。
影响因子：
3.5
作者：
Boehringer,H;Berthold,PH;Taichman,NS
通讯作者：
Taichman,NS