Comparable in vitro Function of Human Liver-Derived and Adipose Tissue-Derived Mesenchymal Stromal Cells: Implications for Cell-Based Therapy.
Comparable in vitro Function of Human Liver-Derived and Adipose Tissue-Derived Mesenchymal Stromal Cells: Implications for Cell-Based Therapy.
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DOI：
10.3389/fcell.2021.641792
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发表时间：
2021
影响因子：
5.5
通讯作者：
Taner T
中科院分区：
文献类型：
--
作者：
Yigitbilek F;Conley SM;Tang H;Saadiq IM;Jordan KL;Lerman LO;Taner T
间充质干细胞/基质细胞（MSCs）因其免疫治疗和再生特性而受到广泛研究，这些特性可能因细胞来源不同而有所差异。在从性别、年龄和体重指数匹配的供体获取的间充质干细胞中，我们比较了肝源性间充质干细胞（L - MSCs）和脂肪组织源性间充质干细胞（A - MSCs）的增殖和迁移功能（每组n = 6名供体）。通过衰老相关β - 半乳糖苷酶活性、利用实时定量聚合酶链反应检测衰老相关分泌表型（SASP）因子的表达以及蛋白质免疫印迹分析来评估细胞衰老情况。通过将间充质干细胞与受损的人脐静脉内皮细胞（HUVEC）共培养，比较间充质干细胞的促血管生成和修复能力。L - MSCs和A - MSCs的增殖与迁移特性相似。尽管两种间充质干细胞中细胞周期停滞和SASP基因的表达相似，但L - MSCs中肿瘤坏死因子α基因和蛋白的表达显著下调。在与受损HUVEC共培养时，A - MSCs比L - MSCs能显著修复更多的血管管腔和管腔连接。因此，尽管L - MSCs和A - MSCs在许多功能上相似，但L - MSCs具有更强的免疫调节特性，而A - MSCs似乎具有更好的促血管生成和血管修复能力。广泛的细胞选择可能性或许能够实现为特定基础疾病挑选合适的细胞疗法。
Mesenchymal stem/stromal cells (MSCs) have been investigated extensively for their immunotherapeutic and regenerative properties, which may differ by cell source. In MSCs harvested from donors matched for sex, age, and body mass index, we compared the proliferative and migration functions of liver-derived MSCs (L-MSCs) and adipose tissue-derived MSCs (A-MSCs) (n = 6 donors each). Cellular senescence was evaluated by senescence-associated beta-galactosidase enzyme activity and expression of senescence-associated secretory phenotype (SASP) factors using real-time quantitative polymerase chain and by western blot assay. The pro-angiogenic and reparative potency of MSCs was compared by co-culturing MSCs with injured human umbilical vein endothelial cells (HUVEC). The proliferation and migration properties were similar in L-MSCs and A-MSCs. Although cell cycle arrest and SASP genes were similarly expressed in both MSCs, tumor necrosis factor alpha gene and protein expression were significantly downregulated in L-MSCs. In co-cultured injured HUVEC, A-MSCs restored significantly more tubes and tube connections than L-MSCs. Therefore, despite many functional similarities between L-MSCs and A-MSCs, L-MSCs have enhanced immunomodulatory properties, while A-MSCs appear to have better pro-angiogenic and vascular reparative potency. Availability of a broad range of cellular options might enable selecting cell-based therapy appropriate for the specific underlying disease.
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DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
影响因子：
4.1
作者：
Kozlowska, Urszula;Krawczenko, Agnieszka;Klimczak, Aleksandra
通讯作者：
Klimczak, Aleksandra
影响因子：
3.5
作者：
Chapel, A;Bertho, JM;Thierry, D
通讯作者：
Thierry, D
影响因子：
5.2
作者：
Baxter, MA;Wynn, RF;Bellantuono, I
通讯作者：
Bellantuono, I
影响因子：
37.8
作者：
Barbash, IM;Chouraqui, P;Leor, J
通讯作者：
Leor, J