Interaction of iPSC-derived neural stem cells on poly(L-lactic acid) nanofibrous scaffolds for possible use in neural tissue engineering.
Interaction of iPSC-derived neural stem cells on poly(L-lactic acid) nanofibrous scaffolds for possible use in neural tissue engineering.
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iPSC 衍生的神经干细胞在聚（L-乳酸）纳米纤维支架上的相互作用，可能用于神经组织工程
DOI：
10.3892/ijmm.2017.3299
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发表时间：
2018-03
影响因子：
5.4
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Lin C;Liu C;Zhang L;Huang Z;Zhao P;Chen R;Pang M;Chen Z;He L;Luo C;Rong L;Liu B
组织工程是一个快速发展的技术领域，用于中枢神经系统损伤的再生和重建。通过将种子细胞与合适的生物材料支架相结合，组织工程具有促进神经再生和功能恢复的能力。本研究以小鼠胚胎成纤维细胞（MEFs）为原核细胞，分别构建非整合型附加型载体pCEP 4-EO 2S-ET 2K和pCEP 4-miR-302-367，诱导小鼠多能干细胞（iPSCs）分化为神经干细胞（NSCs）作为移植细胞。然后使用静电纺丝制备随机取向的聚（L-乳酸）（PLLA）纳米纤维和排列的PLLA纳米纤维，并评估它们与iPSC衍生的NSC（iNSC）的细胞相容性和神经突引导作用。结果表明，非整合的iPSC有效地产生并分化成iNSC。PLLA支架能够促进iNSCs的粘附、生长、存活和增殖。此外，与随机取向的PLLA纳米纤维相比，排列的PLLA纳米纤维极大地引导神经突从iNSC中生长，并显著促进神经突沿纳米纤维排列沿着生长。总体而言，这些发现表明PLLA纳米纤维支架与iNSC在体外联合使用的可行性，并支持其在神经组织工程中的应用潜力。
Tissue engineering is a rapidly growing technological area for the regeneration and reconstruction of damage to the central nervous system. By combining seed cells with appropriate biomaterial scaffolds, tissue engineering has the ability to improve nerve regeneration and functional recovery. In the present study, mouse induced pluripotent stem cells (iPSCs) were generated from mouse embryonic fibroblasts (MEFs) with the non-integrating episomal vectors pCEP4-EO2S-ET2K and pCEP4-miR-302-367 cluster, and differentiated into neural stem cells (NSCs) as transplanting cells. Electrospinning was then used to fabricate randomly oriented poly(L-lactic acid) (PLLA) nanofibers and aligned PLLA nanofibers and assessed their cytocompatibility and neurite guidance effect with iPSC-derived NSCs (iNSCs). The results demonstrated that non-integrated iPSCs were effectively generated and differentiated into iNSCs. PLLA nanofiber scaffolds were able to promote the adhesion, growth, survival and proliferation of the iNSCs. Furthermore, compared with randomly oriented PLLA nanofibers, the aligned PLLA nanofibers greatly directed neurite outgrowth from the iNSCs and significantly promoted neurite growth along the nanofibrous alignment. Overall, these findings indicate the feasibility of using PLLA nanofiber scaffolds in combination with iNSCs in vitro and support their potential for use in nerve tissue engineering.
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影响因子：
5.3
作者：
Nichols, CM;Brenner, MJ;Mackinnon, SE
通讯作者：
Mackinnon, SE
影响因子：
5.2
作者：
Hu, Shijun;Wilson, Kitchener D.;Ghosh, Zhumur;Han, Leng;Wang, Yongming;Lan, Feng;Ransohoff, Katherine J.;Burridge, Paul;Wu, Joseph C.
通讯作者：
Wu, Joseph C.
影响因子：
3.7
作者：
Kobayashi Y;Okada Y;Itakura G;Iwai H;Nishimura S;Yasuda A;Nori S;Hikishima K;Konomi T;Fujiyoshi K;Tsuji O;Toyama Y;Yamanaka S;Nakamura M;Okano H
通讯作者：
Okano H
影响因子：
5.4
作者：
Liu, Guibo;Cheng, Yongxia;Tong, Xiaojie
通讯作者：
Tong, Xiaojie
影响因子：
3.4
作者：
Darsalia, Vladimer;Kallur, Therese;Kokaia, Zaal
通讯作者：
Kokaia, Zaal