GmPHR25, a GmPHR member up-regulated by phosphate starvation, controls phosphate homeostasis in soybean.
GmPHR25, a GmPHR member up-regulated by phosphate starvation, controls phosphate homeostasis in soybean.
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GmPHR25 是一个因磷酸盐饥饿而上调的 GmPHR 成员，控制大豆中的磷酸盐稳态
DOI：
10.1093/jxb/erx292
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发表时间：
2017-10-13
影响因子：
6.9
通讯作者：
Miriam G
中科院分区：
文献类型：
--
作者：
Xue YB;Xiao BX;Zhu SN;Mo XH;Liang CY;Tian J;Liao H;Miriam G
GmPHR25是磷信号网络中的一个重要调节因子，在磷饥饿条件下表达上调，并控制大豆体内磷的稳态。磷是植物生长发育的必需营养元素，对植物的生长发育起着重要作用。低磷有效性是作物生产的限制因素，特别是对于豆类作物（例如大豆），其需要额外的磷以通过与根瘤菌的共生关系来维持固氮。尽管磷酸盐饥饿反应1（PHR1）或PHR1样蛋白被认为是几种植物中磷酸盐（Pi）稳态的中心调节因子，但它在大豆中仍不明确。本研究从大豆基因组中克隆了35个GmPHR成员，并在缺氮和缺磷条件下分析了它们在大豆中的表达模式。然后，在大豆毛状根中在体外和体内过量表达响应于Pi饥饿而上调的GmPHR25，以研究其功能。结果表明，在正常条件下，过量表达GmPHR25可提高转基因大豆毛状根中的磷浓度，但同时显著降低了毛状根的生长。此外，11的14个高亲和力的Pi转运蛋白（GmPT）的成员，以及其他五个磷饥饿响应基因的转录显着增加，在大豆毛状根与GmPHR25过表达。综上所述，本研究表明GmPHR25是P信号网络中的重要调节剂，并控制大豆中的Pi稳态。
GmPHR25, which is up-regulated by phosphate starvation, is a vital regulator in the phosphorus signaling network, and controls phosphate homeostasis in soybean. As an essential nutrient element, phosphorus (P) plays an important role in plant growth and development. Low P availability is a limiting factor for crop production, especially for legume crops (e.g. soybean), which require additional P to sustain nitrogen fixation through symbiotic associations with rhizobia. Although PHOSPHATE STARVATION RESPONSE 1 (PHR1) or PHR1-like is considered as a central regulator of phosphate (Pi) homeostasis in several plant species, it remains undefined in soybean. In this study, 35 GmPHR members were cloned from the soybean genome and expression patterns in soybean were assayed under nitrogen (N) and P deficiency conditions. GmPHR25, which is up-regulated in response to Pi starvation, was then overexpressed in soybean hairy roots in vitro and in vivo to investigate its functions. The results showed that overexpressing GmPHR25 increased Pi concentration in transgenic soybean hairy roots under normal conditions, accompanied with a significant decrease in hairy root growth. Furthermore, transcripts of 11 out of 14 high-affinity Pi transporter (GmPT) members as well as five other Pi starvation-responsive genes were significantly increased in soybean hairy roots with GmPHR25 overexpression. Taken together, this study suggests that GmPHR25 is a vital regulator in the P signaling network, and controls Pi homeostasis in soybean.
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