Effect of loop length variation on quadruplex-Watson Crick duplex competition.
Effect of loop length variation on quadruplex-Watson Crick duplex competition.
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DOI：
10.1093/nar/gkn402
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发表时间：
2008-08
影响因子：
14.9
通讯作者：
Maiti S
中科院分区：
文献类型：
--
作者：
Kumar N;Sahoo B;Varun KA;Maiti S;Maiti S
当富G链与其互补的富C链同时存在时，研究了环长对四链稳定性的影响，从而导致四链和双链结构之间的竞争。使用环长从T到T5的模型序列，我们进行了广泛的FRET，以发现环长对四重-Watson Crick双工竞争的影响。结合数据显示，随着环长的增加，四链体与其互补链的结合亲和力增加。我们的动力学数据表明，在互补链存在的情况下，四链的展开涉及到主要的慢速和少数快速打开构象的贡献。随着环长度的增加，来自快速打开构象的贡献增加，从而导致更快的双链形成。FCS数据显示，在补充链的存在下，四链构象之间的相互转化增加，随着环长的增加，平衡向快速开放构象移动。双链和四链之间的相对自由能差(ΔΔG°)表明，环长的增加有利于双链的形成，并超过四链的竞争。
The effect of loop length on quadruplex stability has been studied when the G-rich strand is present along with its complementary C-rich strand, thereby resulting in competition between quadruplex and duplex structures. Using model sequences with loop lengths varying from T to T5, we carried out extensive FRET to discover the influence of loop length on the quadruplex-Watson Crick duplex competition. The binding data show an increase in the binding affinity of quadruplexes towards their complementary strands upon increasing the loop length. Our kinetic data reveal that unfolding of the quadruplex in presence of a complementary strand involves a contribution from a predominant slow and a small population of fast opening conformer. The contribution from the fast opening conformer increases upon increasing the loop length leading to faster duplex formation. FCS data show an increase in the interconversion between the quadruplex conformers in presence of the complementary strand, which shifts the equilibrium towards the fast opening conformer with an increase in loop length. The relative free-energy difference (ΔΔG°) between the duplex and quadruplex indicates that an increase in loop length favors duplex formation and out competes the quadruplex.
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影响因子：
14.9
作者：
Kumar N;Maiti S
通讯作者：
Maiti S
DOI：
10.1016/j.bbrc.2004.05.052
发表时间：
2004-07-02
影响因子：
3.1
作者：
Kumar, N;Maiti, S
通讯作者：
Maiti, S
影响因子：
3.3
作者：
Kumar, Niti;Maiti, Souvik
通讯作者：
Maiti, Souvik
影响因子：
3.9
作者：
Guedin, Aurore;De Cian, Anne;Mergny, Jean-Louis
通讯作者：
Mergny, Jean-Louis
影响因子：
2.9
作者：
FANG, GW;CECH, TR
通讯作者：
CECH, TR