Scavenger Receptor Class B, Type I, a CD36 Related Protein in Macrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sources.
Scavenger Receptor Class B, Type I, a CD36 Related Protein in Macrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sources.
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DOI：
10.1155/2016/6325927
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发表时间：
2016
影响因子：
2.9
通讯作者：
Ye J
中科院分区：
文献类型：
--
作者：
Ding Z;Luo N;Kong Y;Li J;Zhang Y;Cao F;Ye J
清道夫受体B类I型（SR-BI）是包含参与哺乳动物和鱼脂质稳态调节的跨膜蛋白的CD 36超家族的成员。我们推测该受体在日本沼虾脂质代谢中起重要作用。然而，很少有人注意到SR-BI在商业甲壳类动物。在本研究中，我们报告了一个cDNA编码M。日本对虾清道夫受体B类，I型（命名为MnSR-BI），从肝胰腺cDNA文库获得。MnSR-BI基因全长1545 bp，编码514个氨基酸。MnSR-BI一级结构由CD 36结构域组成，该结构域在蛋白质的N-和C-末端含有两个跨膜区。SR-BI mRNA在肌肉、鳃、卵、肠、肝胰腺、胃和卵巢组织中特异表达。此外，其在肝胰腺中的表达受膳食脂肪源的调节，其中饲喂大豆油和亚麻籽油的对虾表现出较高的表达水平。基于RNAi的SR-BI沉默导致其在肝胰腺中的表达抑制和脂质代谢相关基因表达的变化。这是淡水对虾中SR-BI的首次报道，为进一步研究甲壳类动物中SR-BI提供了基础。
The scavenger receptor class B, type I (SR-BI), is a member of the CD36 superfamily comprising transmembrane proteins involved in mammalian and fish lipid homeostasis regulation. We hypothesize that this receptor plays an important role in Macrobrachium nipponense lipid metabolism. However, little attention has been paid to SR-BI in commercial crustaceans. In the present study, we report a cDNA encoding M. nipponense scavenger receptor class B, type I (designated as MnSR-BI), obtained from a hepatopancreas cDNA library. The complete MnSR-BI coding sequence was 1545 bp, encoding 514 amino acid peptides. The MnSR-BI primary structure consisted of a CD36 domain that contained two transmembrane regions at the N- and C-terminals of the protein. SR-BI mRNA expression was specifically detected in muscle, gill, ovum, intestine, hepatopancreas, stomach, and ovary tissues. Furthermore, its expression in the hepatopancreas was regulated by dietary lipid sources, with prawns fed soybean and linseed oils exhibiting higher expression levels. RNAi-based SR-BI silencing resulted in the suppression of its expression in the hepatopancreas and variation in the expression of lipid metabolism-related genes. This is the first report of SR-BI in freshwater prawns and provides the basis for further studies on SR-BI in crustaceans.
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影响因子：
4.8
作者：
Connelly, MA;Klein, SM;Williams, DL
通讯作者：
Williams, DL
影响因子：
15.9
作者：
Landschulz, KT;Pathak, RK;Hobbs, HH
通讯作者：
Hobbs, HH
影响因子：
3.5
作者：
Ding, Z. L.;Chen, L. Q.;Kong, Y. Q.
通讯作者：
Kong, Y. Q.
影响因子：
2
作者：
Li, Jiayao;Guo, Zhanlin;Zhao, Yunlong
通讯作者：
Zhao, Yunlong
影响因子：
5.6
作者：
MANDRUP, S;HUMMEL, R;KRISTIANSEN, K
通讯作者：
KRISTIANSEN, K