Analysis of imprinted gene expression in normal fertilized and uniparental preimplantation porcine embryos.
Analysis of imprinted gene expression in normal fertilized and uniparental preimplantation porcine embryos.
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DOI：
10.1371/journal.pone.0022216
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lee CK
中科院分区：
文献类型：
--
作者：
Park CH;Uh KJ;Mulligan BP;Jeung EB;Hyun SH;Shin T;Ka H;Lee CK
在本研究中，使用定量实时PCR来确定正常受精和单亲猪胚胎在植入前发育过程中八个印记基因（GRB10、H19、IGF2R、XIST、IGF2、NNAT、PEG1和PEG10）的表达状态。结果表明，在所有观察到的胚胎样本中，直到 16 细胞发育阶段，大多数基因都存在非印记基因表达模式。对于所有类别的胚胎来说都是如此，无论亲本起源和印记方向如何。然而，在囊胚发育阶段的类别中检测到了几个差异表达的基因（H19、IGF2、XIST 和 PEG10）。最有趣的是，尽管母本和父本表达的基因不应分别在雄激素和单性生殖酮中表达，但在本研究中进行测试时，两个单亲胚胎都表达了这些基因。为了解释这种现象，我们比较了单倍体和二倍体孤雌生殖胚胎中八个印记基因的表达模式以及 IGF2/H19 DMR3 的甲基化状态。我们的研究结果表明，IGF2、NNAT 和 PEG10 在单倍体而非二倍体孤雌生殖囊胚中被沉默，并且在单倍体和二倍体孤雌生殖囊胚之间一致观察到 IGF2/H19 DMR3 的差异甲基化。这些结果似乎表明，存在一个调节二倍体孤雌胚胎中印记基因表达状态的过程，并且这种现象可能与印记控制区域的甲基化改变有关。此外，我们认为印记表达发生在猪囊胚期胚胎中的至少四个基因中，即H19、IGF2、XIST和PEG10。
In the present study quantitative real-time PCR was used to determine the expression status of eight imprinted genes (GRB10, H19, IGF2R, XIST, IGF2, NNAT, PEG1 and PEG10) during preimplantation development, in normal fertilized and uniparental porcine embryos. The results demonstrated that, in all observed embryo samples, a non imprinted gene expression pattern up to the 16-cell stage of development was common for most genes. This was true for all classes of embryo, regardless of parental-origins and the direction of imprint. However, several differentially expressed genes (H19, IGF2, XIST and PEG10) were detected amongst the classes at the blastocyst stage of development. Most interestingly and despite the fact that maternally and paternally expressed genes should not be expressed in androgenones and parthenogenones, respectively, both uniparental embryos expressed these genes when tested for in this study. In order to account for this phenomenon, we compared the expression patterns of eight imprinted genes along with the methylation status of the IGF2/H19 DMR3 in haploid and diploid parthenogenetic embryos. Our findings revealed that IGF2, NNAT and PEG10 were silenced in haploid but not diploid parthenogenetic blastocysts and differential methylation of the IGF2/H19 DMR3 was consistently observed between haploid and diploid parthenogenetic blastocysts. These results appear to suggest that there exists a process to adjust the expression status of imprinted genes in diploid parthenogenetic embryos and that this phenomenon may be associated with altered methylation at an imprinting control region. In addition we believe that imprinted expression occurs in at least four genes, namely H19, IGF2, XIST and PEG10 in porcine blastocyst stage embryos.
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影响因子：
4.8
作者：
Imamura, T;Kerjean, A;Pàldi, A
通讯作者：
Pàldi, A
DOI：
10.1073/pnas.1532175100
发表时间：
2003-07-08
影响因子：
11.1
作者：
Charalambous, M;Smith, FM;Ward, A
通讯作者：
Ward, A
影响因子：
3.6
作者：
Lagutina, I;Lazzari, G;Galli, C
通讯作者：
Galli, C
影响因子：
2.2
作者：
Park, Chi-Hun;Lee, Sang-Goo;Lee, Chang-Kyu
通讯作者：
Lee, Chang-Kyu
影响因子：
10.5
作者：
LATHAM, KE;DOHERTY, AS;SCHULTZ, RM
通讯作者：
SCHULTZ, RM