Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance.
Clinical assessment of a low-cost, hand-held, smartphone-attached intraoral imaging probe for 5-aminolevulinic acid photodynamic therapy monitoring and guidance.
复制标题
DOI：
10.1117/1.jbo.28.8.082809
复制
发表时间：
2023-08
影响因子：
3.5
通讯作者：
中科院分区：
文献类型：
--
作者：
印度是世界上口腔鳞状细胞癌(OSCC)发病率最高的国家之一，发病率为每10万人中有15人，每年有超过7万人死亡。由于缺乏医疗基础设施和常规筛查，特别是在农村地区，这一问题更加严重。迫切需要新的技术来检测口腔癌并在护理时及时进行治疗。我们的研究目的是使用手持智能手机耦合的口腔内成像设备，以前研究过的自发荧光(Auto-FL)诊断适用于治疗指导和监测光动力疗法(PDT)使用5-氨基乙酰丙酸(ALA)诱导的原卟啉IX(PpIX)荧光(FL)。12例口腔黏膜病变患者14个口腔黏膜病变为中/高分化口腔鳞癌(直径和深度)，系统地给予ALA(共3个剂量)。用多通道FL和偏振白光口腔癌成像探头在ALA给药前后和投光后(分次，共635 nm红色LED光)对病变部位PpIX和Auto-FL进行成像。手持设备有利于获取口腔内病变部位的图像。分割PPIX FL相对于AUTO-FL的比率测量图像能够改善相对于单独PPIX的病变边界的划分。在轻度到重度不典型增生恶性肿瘤患者中，发现相对FL(-值)阈值为1.4来分割病变部位PpIX的产生。节段性病变大小与超声表现有很好的相关性。治疗时-值为-的病变与成功的结果相关。这些结果表明，低成本的手持口腔内成像探头用于图像引导的PDT和治疗监测，同时也为综合方法奠定了基础，将癌症筛查和治疗与相同的硬件相结合。
India has one of the highest rates of oral squamous cell carcinoma (OSCC) in the world, with an incidence of 15 per 100,000 and more than 70,000 deaths per year. The problem is exacerbated by a lack of medical infrastructure and routine screening, especially in rural areas. New technologies for oral cancer detection and timely treatment at the point of care are urgently needed. Our study aimed to use a hand-held smartphone-coupled intraoral imaging device, previously investigated for autofluorescence (auto-FL) diagnostics adapted here for treatment guidance and monitoring photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) fluorescence (FL). A total of 12 patients with 14 buccal mucosal lesions having moderately/well-differentiated micro-invasive OSCC lesions ( diameter and depth) were systemically (in oral solution) administered three doses of ALA (total ). Lesion site PpIX and auto-FL were imaged using the multichannel FL and polarized white-light oral cancer imaging probe before/after ALA administration and after light delivery (fractionated, total of 635 nm red LED light). The handheld device was conducive for access to lesion site images in the oral cavity. Segmentation of ratiometric images in which PpIX FL is mapped relative to auto-FL enabled improved demarcation of lesion boundaries relative to PpIX alone. A relative FL (-value) threshold of 1.4 was found to segment lesion site PpIX production among the patients with mild to severe dysplasia malignancy. The segmented lesion size is well correlated with ultrasound findings. Lesions for which -value was at the time of treatment were associated with successful outcomes. These results indicate the utility of a low-cost, handheld intraoral imaging probe for image-guided PDT and treatment monitoring while also laying the groundwork for an integrated approach, combining cancer screening and treatment with the same hardware.
DOI：
10.3390/s21093219
发表时间：
2021-05-06
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Yan YJ;Cheng NL;Jan CI;Tsai MH;Chiou JC;Ou-Yang M
通讯作者：
Ou-Yang M
基于光的非侵入性诊断系统，用于检测早期口腔癌和高风险癌前病变 - 牙科潜力。
影响因子：
5.2
作者：
Tatehara S;Satomura K
通讯作者：
Satomura K