Volumetric capillary cytometry: a new method for absolute cell enumeration.
Volumetric capillary cytometry: a new method for absolute cell enumeration.
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体积毛细管细胞计数：绝对细胞计数的新方法。
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
N. Sizto
中科院分区：
文献类型：
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作者：
L. Dietz;Robert S. Dubrow;Bala S. Manian;N. Sizto
本文描述了一种新型的细胞计数系统，该系统能够对精确毛细管中包含的精确已知体积的未裂解全血中的荧光标记细胞群体进行成像、识别和计数。全血与针对细胞表面抗原的染料标记抗体反应。红色激发源（氦氖激光器）用于最大限度地减少自体荧光和血液成分的干扰。描述了一种双色荧光检测系统，使用染料Cy 5和Cy5.5，允许同时检测和定量两种不同类型的表面抗原。图像处理技术用于识别图像中的细胞事件并定量两个颜色平面中的荧光强度。通过在全血中进行绝对CD 3 +/CD 4+淋巴细胞计数证明了该技术的实用性，样品制备最少，血液样品完全包含在一次性检测盒中。将该技术与流式细胞术进行比较的相关性研究给出了0.98的相关系数。此外，该系统的能力，以监测反应动力学证明了与抗CD 4抗体到CD 4+淋巴细胞的表面上的关联率的测量。
This article describes a novel cytometry system that is capable of imaging, identifying, and counting populations of fluorescently labeled cells within a precisely known volume of unlysed whole blood contained in a precision capillary. The whole blood is reacted with dye-labeled antibodies directed at cell surface antigens. A red excitation source (helium-neon laser) is used to minimize auto-fluorescence and interference by blood components. A two-color fluorescence detection system is described, using the dyes Cy5 and Cy5.5, allowing the simultaneous detection and quantitation of two different types of surface antigens. Image processing techniques are used to identify cellular events in the image and to quantitate the fluorescent intensities in both color planes. The utility of this technique is demonstrated by performing absolute CD3+/CD4+ lymphocyte counts in whole blood, with minimal sample preparation, and with the blood sample completely contained in a disposable cartridge. A correlation study comparing this technique to flow cytometry gives a correlation coefficient of 0.98. In addition, the ability of this system to monitor reaction kinetics is demonstrated with a measurement of the rate of association of the anti-CD4 antibody onto the surface of CD4+ lymphocytes.
从 CD4 百分比预测 CD4 计数：来自三个实验室的经验。
DOI：
10.1097/00002030-199307000-00005
发表时间：
1993
期刊：
AIDS (London, England)
影响因子：
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作者：
Kidd,PG;Cheng,SC;Paxton,H;Landay,A;Gelman,R
通讯作者：
Gelman,R
评估确定 CD4 淋巴细胞绝对计数的四种替代方法。
DOI：
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发表时间：
1995
期刊：
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association
影响因子：
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作者：
Johnson,D;Hirschkorn,D;Busch,MP
通讯作者：
Busch,MP