Low jasmonic acid accumulation causes the defect in the tepal expansion in double-flowered green lily cultivar, ‘Doubleen’
Low jasmonic acid accumulation causes the defect in the tepal expansion in double-flowered green lily cultivar, ‘Doubleen’
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茉莉酸积累量低导致重瓣绿百合品种“Doubleen”的花被扩张缺陷
DOI：
10.1016/j.scienta.2022.111539
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发表时间：
2023
影响因子：
4.3
通讯作者：
Yoshikuni Kitamura
中科院分区：
文献类型：
--
作者：
Takuya Fukasawa ; Mikiko Kojima ; Yumiko Takebayashi ; Hitoshi Sakakibara ; Ken ichi Matsushima ; Ken ichiro Yasuba ; Yuichi Yoshida ; Tanjuro Goto ; Yoshikuni Kitamura
本研究以百合品种“双百合”为试材，以茉莉酸（JA）积累为研究对象，探讨了百合绿色花表型的形成机制。“双莲”是从“耶洛温”衍生的突变型百合品种，具有未展开的、无色素的绿色花被片，但缺少雄蕊和心皮。用茉莉酸甲酯（MeJA）处理‘Doubleen’，以研究随后花器官特征的变化。结果，MeJA处理的'Doubleen'的花被片扩大并有黄色色素沉着，而对照没有。为了确定茉莉酸（JA）在百合花中的合成位点，比较了茉莉酸（JA）和茉莉酸-L-异亮氨酸（JA-Ile）在“黄百合”花器官中的积累情况。在所有发育阶段，心皮的JA-Ile含量都是三个花器官中最低的。内花被片和雄蕊中的JA-Ile含量在各发育时期差异不显著。为了确定JA和JA-Ile在百合花花被片扩展和色素沉着中的潜在作用，比较了这两种化合物在'Yelloween'和'Doubleen'花被片之间的积累。在所有发育阶段，'Doubleen'花被片积累的JA浓度低于'Yelloween'。花器官去除实验也进行了使用'Yelloween'，以评估其对花被片扩张的影响。值得注意的是，花被片扩张不受雌蕊去除，但强烈抑制雄蕊去除。这些结果表明，在'Doubleen'的花中JA生物合成位点的丢失导致其花上未展开的绿色花被片的形成。
In this study, the mechanisms underlying green flower phenotype of a lily cultivar, ‘Doubleen’, was investigated focusing on jasmonic acid (JA) accumulation. ‘Doubleen’ is a mutant lily cultivar derived from ‘Yelloween’ and has unexpanded, non-pigmented green tepals but lacks the stamen and carpel. ‘Doubleen’ was treated with methyl jasmonate (MeJA) to investigate the subsequent change in floral organ characteristics. As a result, the tepals of MeJA-treated ‘Doubleen’ expanded and had yellow pigmentation, whereas those of the control did not. To estimate the JA synthesis site in the lily flower, the accumulation profiles of JA and jasmonoyl-L-isoleucine (JA-Ile) were compared between the floral organs of ‘Yelloween’. The JA-Ile content of carpels was the lowest of the three floral organs at all developmental stages. JA-Ile contents in the inner tepals and stamens were not significantly different in all developmental stages. To determine potential role of JA and JA-Ile in tepal expansion and pigmentation in lily flowers, the accumulation of these two compounds was compared between the tepals of ‘Yelloween’ and those of ‘Doubleen’. At all developmental stages, the tepals of ‘Doubleen’ accumulated JA at concentrations lower than those of ‘Yelloween’. Floral organ removal experiments were also conducted using ‘Yelloween’ to evaluate its effect on tepal expansion. Notably, tepal expansion was not affected by pistil removal, but was strongly suppressed by stamen removal. Overall, these results suggest the loss of JA biosynthesis site in the flowers of ‘Doubleen’ leading to the formation of unexpanded green tepals on their flowers.
影响因子：
4.6
作者：
John L. Bowman;David R. Smyth;E. Meyerowitz
通讯作者：
John L. Bowman;David R. Smyth;E. Meyerowitz
影响因子：
4.4
作者：
Suzuki K;Suzuki T;Nakatsuka T;Dohra H;Yamagishi M;Matsuyama K;Matsuura H
通讯作者：
Matsuura H
茉莉酸甲酯可增强黄色切花玫瑰花的颜色和类胡萝卜素含量
DOI：
10.17660/actahortic.2007.755.31
发表时间：
2007
期刊：
影响因子：
--
作者：
A. Glick;S. Philosoph;A. Vainstein;A. Meir;Y. Tadmor;S. Meir
通讯作者：
S. Meir