The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice.
The APETALA-2-like transcription factor OsAP2-39 controls key interactions between abscisic acid and gibberellin in rice.
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DOI：
10.1371/journal.pgen.1001098
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发表时间：
2010-09-09
期刊：
影响因子：
4.5
通讯作者：
Rothstein SJ
中科院分区：
文献类型：
--
作者：
Yaish MW;El-Kereamy A;Zhu T;Beatty PH;Good AG;Bi YM;Rothstein SJ
植物激素之间的相互作用是控制植物生长和发育过程的重要机制。破译这些相互作用是帮助开发提高产量和抵抗环境压力的作物的关键一步。控制包含 APETALA 2 (AP2) 结构域的 OsAP2-39 的表达水平会导致水稻表型变化。 OsAP2-39 的过度表达会减少转基因水稻品系中的生物量和种子数量，从而导致产量下降。 OsAP2-39 过表达转基因水稻的全局转录组分析揭示了关键脱落酸 (ABA) 生物合成基因 OsNCED-I 的上调，该基因编码 9-顺式环氧类胡萝卜素双加氧酶，导致内源 ABA 水平增加。除了 OsNCED-1 之外，基因表达分析还揭示了编码最上节间伸长 (EUI) 蛋白的基因的上调，EUI 是一种催化非 13-羟基化 GA 的 16α、17-环氧化的酶，已被证明可以使水稻中的赤霉素 (GA) 失活。外源应用 GA 可恢复转基因品系中的野生型表型，应用 ABA 可诱导野生型品系中 EUI 的表达并抑制 OsAP2-39 的表达。这些观察结果阐明了 ABA 和 GA 之间的拮抗关系，并说明了导致这些激素稳态的机制。体内和体外分析表明OsNCED-1和EUI的表达均受OsAP2-39直接控制。总之，这些结果揭示了控制水稻 ABA/GA 平衡的新机制，该平衡受 OsAP2-39 调节，进而调节植物生长和种子生产。激素通过一系列动态且复杂的相互作用在控制植物生长和发育方面发挥着重要作用。 ABA 和 GA 是众所周知的拮抗伙伴，尽管其发生机制仍需要进一步阐明。在这个项目中，我们发现从水稻中分离出来的编码 AP2 结构域的转录因子 (OsAP2-39) 直接控制关键的 ABA 生物合成基因 (OsNCED-1) 以及编码 GA 失活蛋白 (EUI) 的基因。此外，我们发现 ABA 诱导 EUI 的表达，进而导致 GA 失活。 ABA 还抑制 OsAP2-39 的表达，从而导致 ABA 合成减少。因此，OsAP2-39 将 ABA 产生和 GA 失活过程联系起来，从而实现 ABA/GA 平衡和稳态。
The interaction between phytohormones is an important mechanism which controls growth and developmental processes in plants. Deciphering these interactions is a crucial step in helping to develop crops with enhanced yield and resistance to environmental stresses. Controlling the expression level of OsAP2-39 which includes an APETALA 2 (AP2) domain leads to phenotypic changes in rice. Overexpression of OsAP2-39 leads to a reduction in yield by decreasing the biomass and the number of seeds in the transgenic rice lines. Global transcriptome analysis of the OsAP2-39 overexpression transgenic rice revealed the upregulation of a key Abscisic Acid (ABA) biosynthetic gene OsNCED-I which codes for 9-cis-epoxycarotenoid dioxygenase and leads to an increase in the endogenous ABA level. In addition to OsNCED-1, the gene expression analysis revealed the upregulation of a gene that codes for the Elongation of Upper most Internode (EUI) protein, an enzyme that catalyzes 16α, 17-epoxidation of non-13-hydroxylated GAs, which has been shown to deactivate gibberellins (GAs) in rice. The exogenous application of GA restores the wild-type phenotype in the transgenic line and ABA application induces the expression of EUI and suppresses the expression of OsAP2-39 in the wild-type line. These observations clarify the antagonistic relationship between ABA and GA and illustrate a mechanism that leads to homeostasis of these hormones. In vivo and in vitro analysis showed that the expression of both OsNCED-1 and EUI are directly controlled by OsAP2-39. Together, these results reveal a novel mechanism for the control of the ABA/GA balance in rice which is regulated by OsAP2-39 that in turn regulates plant growth and seed production. Hormones play an important role in controlling plant growth and development through a dynamic and complicated set of interactions. ABA and GA are well-known as antagonistic partners although the mechanism through which this occurs still needs further elucidation. In this project, we found that a transcription factor isolated from rice and coding for the AP2 domain (OsAP2-39) directly controls a key ABA biosynthetic gene (OsNCED-1) and also a gene that codes for a GA deactivation protein (EUI). In addition, we show that ABA induces the expression of EUI which in turn would lead to GA deactivation. ABA also suppresses OsAP2-39 expression which would lead to a reduction in ABA synthesis. Therefore, OsAP2-39 links the ABA production and GA deactivation processes which results in ABA/GA balance and homeostasis.
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DOI：
10.1073/pnas.96.18.10284
发表时间：
1999-08-31
影响因子：
11.1
作者：
Ashikari, M;Wu, JZ;Yoshimura, A
通讯作者：
Yoshimura, A
影响因子：
7.4
作者：
Curaba, J;Moritz, T;Vachon, G
通讯作者：
Vachon, G
影响因子：
7.2
作者：
Chiwocha, SDS;Cutler, AJ;Kermode, AR
通讯作者：
Kermode, AR
影响因子：
1.4
作者：
Kiseleva, IS;Kaminskaya, OA
通讯作者：
Kaminskaya, OA
影响因子：
5.1
作者：
Johnson, Russell R.;Shin, Margaret;Shen, Jeff Q.
通讯作者：
Shen, Jeff Q.