Regulatory effect of mild moxibustion on P2X3 receptors in spinal cord, anterior cingulate cortex and thalamic ventral posterolateral nucleus of rats with IBS visceral hyperalgesia
Regulatory effect of mild moxibustion on P2X3 receptors in spinal cord, anterior cingulate cortex and thalamic ventral posterolateral nucleus of rats with IBS visceral hyperalgesia
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温和灸对IBS内脏痛过敏大鼠脊髓、前扣带皮层及丘脑腹后外侧核P2X3受体的调节作用
DOI：
10.1007/s11726-021-1254-8
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Cui Yun-hua
中科院分区：
文献类型：
--
作者：
Zhang Zhi-ying;Zhang Fang;Weng Zhi-jun;Wu Huan-gan;Zhou Yun;Han Dong;Li Guo-na;Liu Hui-rong;Cui Yun-hua
观察温和灸对肠易激综合征(IBS)内脏痛觉过敏模型大鼠的治疗效果及其对脊髓、前扣带状皮层(ACC)和丘脑腹侧后外侧核(VPL)P2X3受体的调节作用。将30只8日龄新生大鼠按照完全随机数字表法分为正常组（n=6）和模型组（n=24）。正常组大鼠常规饲养，模型组大鼠采用结直肠扩张（CRD）刺激法制备IBS慢性内脏痛觉过敏模型。将造模成功的大鼠按照完全随机数字表法分为模型组、温和灸组、P2X3受体拮抗剂组、生理盐水组，每组6只。各组大鼠从37日龄开始接受相应的干预，每天一次，连续7天。采用免疫组化和Western blot法检测大鼠脊髓、ACC和VPL中P2X3蛋白的表达。不同强度CRD刺激下，模型组腹部缩回反射（AWR）评分较正常组显着升高（均P<0.05）；温和灸组和P2X3受体拮抗剂组AWR评分较模型组显着降低（均P<0.01）。模型组大鼠脊髓、ACC、VPL组织中P2X3蛋白表达较正常组显着升高（均P<0.01）；温和灸组和P2X3受体拮抗剂组大鼠脊髓、ACC、VPL组织中P2X3蛋白表达量较模型组显着降低（均P<0.01）。温和灸可抑制IBS内脏痛过敏模型大鼠脊髓、ACC、VPL组织中P2X3受体的表达,这可能是温和灸缓解IBS内脏痛过敏大鼠中枢敏化的机制。
To observe the therapeutic effect of mild moxibustion on irritable bowel syndrome (IBS) visceral hyperalgesia model rats and its regulatory effect on P2X3 receptors in the spinal cord, anterior cingutate cortex (ACC) and thalamic ventral posterolateral nucleus (VPL). Thirty 8-day-old newborn rats were randomly divided into a normal group (n=6) and a modeling group (n=24) according to the completely random number table method. Rats in the normal group were bred routinely, and those in the modeling group were subjected to preparing IBS chronic visceral hyperalgesia model using colorectal distention (CRD) in stimulation method. Rats successfully modelled were re-divided into a model group, a mild moxibustion group, a P2X3 receptor antagonist group, and a normal saline group according to the completely random number table method with 6 rats in each group. Rats in each group received corresponding interventions from the 37-day old, once a day for 7 consecutive days. Immunohistochemistry and Western blot assays were used to detect P2X3 protein expressions in the spinal cord, ACC and VPL of rats. Under different intensities of CRD stimulation, the abdominal withdrawal reflex (AWR) scores of the model group were significantly increased versus the normal group (all P<0.05); the AWR scores of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group (all P<0.01). The P2X3 protein expressions in rat spinal cord, ACC and VPL tissues of the model group were significantly increased versus the normal group (all P<0.01); the P2X3 protein expressions in rat spinal cord, ACC and VPL tissues of the mild moxibustion group and the P2X3 receptor antagonist group were significantly reduced versus the model group (all P<0.01). Mild moxibustion can inhibit the P2X3 receptor expressions in the spinal cord, ACC, and VPL tissues of IBS visceral hyperalgesia model rats, which may be the mechanism of mild moxibustion in relieving the central sensitization of rats with IBS visceral hyperalgesia.
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