Immunological response mounted by Aboriginal Australians living in the Northern Territory of Australia against Streptococcus pyogenes serum opacity factor.
Immunological response mounted by Aboriginal Australians living in the Northern Territory of Australia against Streptococcus pyogenes serum opacity factor.
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居住在澳大利亚北领地的澳大利亚原住民对化脓性链球菌血清混浊因子产生的免疫反应。
DOI：
10.1099/00221287-148-1-169
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发表时间：
2002
期刊：
影响因子：
1.5
通讯作者：
M. Walker
中科院分区：
文献类型：
--
作者：
C. Gillen;R. Towers;D. McMillan;Armando Delvecchio;K. S. Sriprakash;B. Currie;B. Kreikemeyer;G. S. Chhatwal;M. Walker
化脓性链球菌（A 组链球菌）通过许多不同的表面成分与宿主纤连蛋白相互作用。链球菌血清混浊因子 (SOF) 是化脓性链球菌的一种细胞表面蛋白，可导致人血清呈乳光并介导细菌与纤连蛋白的结合。在这项研究中，包含全长 SOF 的六组氨酰标签融合蛋白以及包含不透明因子活性和纤连蛋白结合区域的 SOF 结构域，被用于表征原住民对 SOF 的免疫反应。与非原住民成人组相比，原住民成人和儿童的抗 SOF 血清 IgG 反应显着更高 (P<0.0001)。与本研究中检测的针对整个 SOF 蛋白和 N 末端结构域的反应相比，针对 SOF 纤连蛋白结合区的原住民免疫反应显着降低（P<0.001）。在用重组 SOF 免疫的兔子中也观察到了这种免疫反应模式。将来自一些澳大利亚常见分离株的 SOF 推导的氨基酸序列与其他 SOF 序列进行比较，发现 SOF 的 N 末端表现出 42.9% 至 96.5% 的序列相似性值。含有纤连蛋白结合结构域和跨膜区域的 C 末端更加高度保守，在纤连蛋白结合重复序列中表现出 84.6% 至 100% 的序列相似性值。这些数据表明针对 SOF 的免疫反应针对 SOF 蛋白的可变 N 末端。系统发育分析表明化脓性链球菌的sof基因不表现出地理变异。
Streptococcus pyogenes (Group A streptococcus) interacts with host fibronectin via a number of distinct surface components. The streptococcal serum opacity factor (SOF) is a cell-surface protein of S. pyogenes which causes opalescence of human serum and mediates bacterial binding to fibronectin. In this study, hexahistidyl-tagged fusion proteins encompassing full-length SOF, and domains of SOF encompassing opacity factor activity and fibronectin-binding regions, were used in the characterization of the Aboriginal immune response to SOF. Anti-SOF serum IgG responses were found to be significantly higher (P<0.0001) in Aboriginal adults and children when compared to a non-Aboriginal adult group. The Aboriginal immune response against the fibronectin-binding region of SOF was significantly reduced when compared to the response against the whole SOF protein and N-terminal domains examined in this study (P<0.001). This pattern of immune response was also observed in rabbits immunized with recombinant SOF. Comparison of the deduced amino acid sequence of SOF from a number of common Australian isolates with other SOF sequences revealed that the N-terminus of SOF exhibits sequence similarity values ranging from 42.9% to 96.5%. The C-terminus containing the fibronectin-binding domain and membrane-spanning regions was more highly conserved, exhibiting sequence similarity values ranging from 84.6% to 100% within the fibronectin-binding repeats. These data suggest that the immune response against SOF is directed toward the variable N-terminus of the SOF protein. Phylogenetic analysis indicated that the sof genes of S. pyogenes do not exhibit geographical variation.
影响因子：
6.4
作者：
BEACHEY, EH
通讯作者：
BEACHEY, EH