APE-type non-LTR retrotransposons of multicellular organisms encode virus-like 2A oligopeptide sequences, which mediate translational recoding during protein synthesis.
APE-type non-LTR retrotransposons of multicellular organisms encode virus-like 2A oligopeptide sequences, which mediate translational recoding during protein synthesis.
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DOI：
10.1093/molbev/mst102
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发表时间：
2013-08
影响因子：
10.7
通讯作者：
Sukhodub A
中科院分区：
文献类型：
--
作者：
Odon V;Luke GA;Roulston C;de Felipe P;Ruan L;Escuin-Ordinas H;Brown JD;Ryan MD;Sukhodub A
2A寡肽序列（“2A”）介导称为“核糖体跳变”的共翻译重编码事件。之前我们证实了2As（和“2a样序列”）在多种动物RNA病毒基因组中的活性，以及在单细胞生物布氏锥虫（Ingi）和克氏锥虫（L1Tc）基因组中的非长末端重复反转录转座子（non- lts）的活性。在这里，我们报道了2a样序列在广泛的多细胞生物基因组中的存在，并且在锥虫基因组中，在Rex1， Crack, L2， L2A和CR1分支中聚集的非ltr反转录转座子（non- lts）中，除了Ingi之外，还存在2a样序列。对这些2a样序列进行了翻译编码活性测试，在Rex1、L2、CR1和Ingi分支中发现了高度活跃的序列。非ltrs中2a样序列的存在可能不仅代表了一种控制蛋白质生物发生的方法，而且与这种无尿嘧啶/无嘧啶DNA内切酶型非ltrs编码一个而不是两个开放阅读框（orf）有一定的相关性。有趣的是，这类非ltr与密切相关的元素聚集在一起，缺乏类似2a的编码元素，但保留了ORF1。综上所述，这些观察结果表明，获得2a样翻译重编码序列可能在这些元件的进化中发挥了作用。
2A oligopeptide sequences (“2As”) mediate a cotranslational recoding event termed “ribosome skipping.” Previously we demonstrated the activity of 2As (and “2A-like sequences”) within a wide range of animal RNA virus genomes and non-long terminal repeat retrotransposons (non-LTRs) in the genomes of the unicellular organisms Trypanosoma brucei (Ingi) and T. cruzi (L1Tc). Here, we report the presence of 2A-like sequences in the genomes of a wide range of multicellular organisms and, as in the trypanosome genomes, within non-LTR retrotransposons (non-LTRs)—clustering in the Rex1, Crack, L2, L2A, and CR1 clades, in addition to Ingi. These 2A-like sequences were tested for translational recoding activity, and highly active sequences were found within the Rex1, L2, CR1, and Ingi clades. The presence of 2A-like sequences within non-LTRs may not only represent a method of controlling protein biogenesis but also shows some correlation with such apurinic/apyrimidinic DNA endonuclease-type non-LTRs encoding one, rather than two, open reading frames (ORFs). Interestingly, such non-LTRs cluster with closely related elements lacking 2A-like recoding elements but retaining ORF1. Taken together, these observations suggest that acquisition of 2A-like translational recoding sequences may have played a role in the evolution of these elements.
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影响因子：
1.7
作者：
Jurka, J;Kapitonov, VV;Walichiewicz, J
通讯作者：
Walichiewicz, J
DOI：
10.1007/978-0-387-89382-2_5
发表时间：
2010-01-01
期刊：
RECODING: EXPANSION OF DECODING RULES ENRICHES GENE EXPRESSION
影响因子：
--
作者：
Brown, Jeremy D.;Ryan, Martin D.
通讯作者：
Ryan, Martin D.
影响因子：
5.3
作者：
Kojima, KK;Matsumoto, T;Fujiwara, H
通讯作者：
Fujiwara, H
影响因子：
5.4
作者：
Gould, Phillip Spencer;Easton, Andrew John
通讯作者：
Easton, Andrew John
影响因子：
10.5
作者：
Alisch, RS;Garcia-Perez, JL;Moran, JV
通讯作者：
Moran, JV