Biophysical characterization of iron in mitochondria isolated from respiring and fermenting yeast.
Biophysical characterization of iron in mitochondria isolated from respiring and fermenting yeast.
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DOI：
10.1021/bi100558z
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发表时间：
2010-07-06
期刊：
影响因子：
2.9
通讯作者：
Lindahl, Paul A.
中科院分区：
文献类型：
--
作者：
Morales, Jessica Garber;Holmes-Hampton, Gregory P.;Miao, Ren;Guo, Yisong;Muenck, Eckard;Lindahl, Paul A.
采用穆斯堡尔谱、电子吸收光谱、电子顺磁共振和电感耦合等离子体质谱等综合生物物理方法，测定了呼吸、呼吸发酵和发酵酵母细胞线粒体中铁的分布。呼吸细胞线粒体中约40%的铁存在于呼吸相关蛋白中。呼吸发酵线粒体中铁的浓度和分布与呼吸线粒体相似，呼吸作用和发酵同时发生。发酵过程中线粒体中铁的浓度也相似，但分布有很大差异。在这里，与呼吸有关的含铁蛋白减少了约3倍，而非血红素HS FeII物种、非血红素单核HS FeIII和FeIII纳米颗粒占主导地位。这些变化被非血红素HS FeII离子池作为Fe/S簇和血红素生物合成的原料的模型合理化。估计了呼吸相关蛋白复合体的绝对浓度。
The distributions of Fe in mitochondria isolated from respiring, respiro-fermenting, and fermenting yeast cells were determined by an integrative biophysical approach involving Mössbauer and electronic absorption spectroscopies, EPR and ICP-MS. Approximately 40% of the Fe in mitochondria from respiring cells was present in respiration-related proteins. The concentration and distribution of Fe in respiro-fermenting mitochondria, where both respiration and fermentation occur concurrently, was similar to that of respiring mitochondria. The concentration of Fe in fermenting mitochondria was also similar, but the distribution differed dramatically. Here, respiration-related Fe-containing proteins were diminished ca. 3-fold, while nonheme HS FeII species, nonheme mononuclear HS FeIII, and FeIII nanoparticles dominated. These changes were rationalized by a model in which the pool of nonheme HS FeII ions serves as feedstock for Fe/S cluster and heme biosynthesis. The absolute concentrations of respiration-related protein complexes were estimated.
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影响因子：
2.9
作者：
Holmes-Hampton, Gregory P.;Miao, Ren;Lindahl, Paul A.
通讯作者：
Lindahl, Paul A.
影响因子：
7.4
作者：
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影响因子：
11.4
作者：
Dabir, Deepa V.;Leverich, Edward P.;Koehler, Carla M.
通讯作者：
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影响因子：
4.3
作者：
Liao, GL;Palmer, G
通讯作者：
Palmer, G
影响因子：
4.8
作者：
Cobine, PA;Ojeda, LD;Winge, DR
通讯作者：
Winge, DR