Reference point indentation is not indicative of whole mouse bone measures of stress intensity fracture toughness.
Reference point indentation is not indicative of whole mouse bone measures of stress intensity fracture toughness.
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参考点的压痕并不表示全鼠骨骼的应力强度骨折韧性的测量。
DOI：
10.1016/j.bone.2014.09.020
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发表时间：
2014-12
期刊：
影响因子：
4.1
通讯作者：
Shefelbine, Sandra J.
中科院分区：
文献类型：
--
作者：
Carriero, Alessandra;Bruse, Jan L.;Oldknow, Karla J.;Millan, Jose Luis;Farquharson, Colin;Shefelbine, Sandra J.
骨脆性是老年和患病骨的一个问题。测量骨韧性和了解骨的骨折特性对于预测与年龄和疾病相关的骨折风险以及治疗的临床前测试至关重要。最近开发了一种参考点压痕技术（BioDent），通过测量相同位置上第一个和最后一个压痕之间的压痕距离增加（IDI），以微创方式确定骨的抗骨折性。在这项研究中，我们研究了断裂韧性KC和参考点压痕参数（即IDI，总压痕距离（TID）和蠕变压痕距离（CID））之间的关系，来自6种类型（C57 Bl/6，Balb，oim/oim，oim/+，Phospho 1-/-和Phospho 1野生型对应物）的38只小鼠的骨骼。这些小鼠骨骼是健康和患病骨骼的模型，其断裂韧性范围从非常脆（oim/oim）到韧性（Phospho 1 −/−）。切开左侧股骨，开槽并进行3点弯曲试验，直至完全失效。在10个压痕周期内，解剖并压痕股骨干前后表面的10个部位。测定IDI、TID和CID。这项研究的结果表明，参考点压痕参数并不表示在小鼠骨的应力强度断裂韧性。特别是，IDI值在前中骨干跨小鼠类型重叠，使得难以辨别小鼠类型之间的差异，尽管有极端的差异，在应力强度为基础的韧性措施。当考虑更多的压痕位置时，归一化的IDIs可以区分小鼠类型。未来的研究应探讨小鼠骨的参考点压痕参数与骨组织其他材料特性的关系，以确定其用于测量骨质量。我们研究了六种不同小鼠骨类型的断裂韧性和BioDent微压痕参考点压痕参数。断裂韧性明确区分小鼠表型。不同类型小鼠骨干中段的BioDent参数彼此接近，因此难以区分各组。增加沿着骨干的压痕数量会增加反映骨异质性的数据内的标准差。BioDent参数值并不能指示小鼠骨的应力强度断裂韧性。
Bone fragility is a concern for aged and diseased bone. Measuring bone toughness and understanding fracture properties of the bone are critical for predicting fracture risk associated with age and disease and for preclinical testing of therapies. A reference point indentation technique (BioDent) has recently been developed to determine bone's resistance to fracture in a minimally invasive way by measuring the indentation distance increase (IDI) between the first and last indentations over cyclic indentations in the same position. In this study, we investigate the relationship between fracture toughness KC and reference point indentation parameters (i.e. IDI, total indentation distance (TID) and creep indentation distance (CID)) in bones from 38 mice from six types (C57Bl/6, Balb, oim/oim, oim/+, Phospho1−/− and Phospho1 wild type counterpart). These mice bone are models of healthy and diseased bone spanning a range of fracture toughness from very brittle (oim/oim) to ductile (Phospho1−/−). Left femora were dissected, notched and tested in 3-point bending until complete failure. Contralateral femora were dissected and indented in 10 sites of their anterior and posterior shaft surface over 10 indentation cycles. IDI, TID and CID were measured. Results from this study suggest that reference point indentation parameters are not indicative of stress intensity fracture toughness in mouse bone. In particular, the IDI values at the anterior mid-diaphysis across mouse types overlapped, making it difficult to discern differences between mouse types, despite having extreme differences in stress intensity based toughness measures. When more locations of indentation were considered, the normalised IDIs could distinguish between mouse types. Future studies should investigate the relationship of the reference point indentation parameters for mouse bone in other material properties of the bone tissue in order to determine their use for measuring bone quality. We investigated fracture toughness and reference point indentation parameters from BioDent microindentation in six different mouse bone types. Fracture toughness clearly distinguished mouse phenotypes. BioDent parameters at mid-diaphysis across mouse types were close to each other making it difficult to discern between groups. Increasing the number of indentations along the bone shaft increases the standard deviations within the data reflecting bone's heterogeneity. BioDent parameters values are not indicative of stress intensity fracture toughness in mouse bone.
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DOI：
10.1016/j.jmbbm.2008.10.008
发表时间：
2009-08-01
影响因子：
3.9
作者：
Kruzic, J. J.;Kim, D. K.;Ritchie, R. O.
通讯作者：
Ritchie, R. O.
影响因子：
41.2
作者：
Peterlik, H;Roschger, P;Fratzl, P
通讯作者：
Fratzl, P
影响因子：
4.1
作者：
Beamer, WG;Donahue, LR;Baylink, DJ
通讯作者：
Baylink, DJ
影响因子：
4.1
作者：
Huesa, Carmen;Yadav, Manisha C.;Farquharson, Colin
通讯作者：
Farquharson, Colin
影响因子：
6.2
作者：
Diez-Perez, Adolfo;Guerri, Roberto;Nogues, Xavier;Caceres, Enric;Jesus Pena, Maria;Mellibovsky, Leonardo;Randall, Connor;Bridges, Daniel;Weaver, James C.;Proctor, Alexander;Brimer, Davis;Koester, Kurt J.;Ritchie, Robert O.;Hansma, Paul K.
通讯作者：
Hansma, Paul K.