Amelioration of insulin resistance in rat cells by Astragalus polysaccharides and associated mechanisms.
Amelioration of insulin resistance in rat cells by Astragalus polysaccharides and associated mechanisms.
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DOI：
10.3892/etm.2014.1626
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发表时间：
2014-06
影响因子：
2.7
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Liu H;Bai J;Weng X;Wang T;Li M
本研究旨在探讨黄芪多糖（Astragalus polysaccharides， APS）改善大鼠细胞胰岛素抵抗（insulin resistance， IR）的作用及其机制。将完全分化、诱导的3T3-L1大鼠脂肪细胞分为对照组和3个干预组。干预组分别在含有0.001、0.1和10 μg/μl APS的培养基中孵育48 h。处理后，采用酶联免疫吸附法检测培养脂肪细胞分泌的白细胞介素-6和脂联素水平。中等浓度干预组3T3-L1脂肪细胞分泌脂联素水平较对照组显著升高（P<0.05），低、高浓度干预组3T3-L1脂肪细胞分泌脂联素水平较对照组降低（P<0.05、P<0.05）。3个干预组3T3-L1脂肪细胞分泌IL-6水平均低于对照组（低、中、高浓度干预组分别为P<0.05， P<0.05， P<0.05），且呈剂量依赖性。结果表明，黄芪多糖能够增加3T3-L1大鼠脂肪细胞脂联素分泌，降低IL-6分泌，且呈剂量依赖性。这些发现可能确定使用APS改善IR的潜在机制。
The aim of this study was to investigate the function of Astragalus polysaccharides (APS) in ameliorating insulin resistance (IR) in rat cells and to elucidate the associated mechanisms. Fully differentiated, induced 3T3-L1 rat adipocytes were divided into a control group and three intervention groups. The intervention groups were incubated in media containing 0.001, 0.1 and 10 μg/μl APS, respectively, for 48 h. Following treatment, levels of interleukin (IL)-6 and adiponectin secreted by the cultured adipocytes were measured using enzyme-linked immunosorbent assay. Levels of adiponectin secreted by the 3T3-L1 adipocytes in the moderate-concentration intervention group were significantly increased compared with those in the control group (P<0.05), whereas levels of adiponectin secreted by the 3T3-L1 adipocytes in the low- and high-concentration intervention groups were decreased compared with those in the control group (P<0.05 and P>0.05, respectively). Levels of IL-6 secreted by the 3T3-L1 adipocytes in the three intervention groups were lower than those in the control group (P>0.05, P<0.05 and P<0.05 for the low- moderate- and high-concentration intervention groups, respectively), and demonstrated APS dose-dependence. The results indicate that APS are capable of increasing adiponectin secretion and reducing IL-6 secretion by 3T3-L1 rat adipocytes in a dose-dependent manner. These findings may identify a potential mechanism for ameliorating IR using APS.
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