Holographic photolysis of caged neurotransmitters.
Holographic photolysis of caged neurotransmitters.
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DOI：
10.1038/nmeth.1241
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发表时间：
2008-09
期刊：
影响因子：
48
通讯作者：
Emiliani, Valentina
中科院分区：
文献类型：
--
作者：
Lutz, Christoph;Otis, Thomas S.;DeSars, Vincent;Charpak, Serge;DiGregorio, David A.;Emiliani, Valentina
Stimulation of light-sensitive chemical probes has become a powerful tool for the study of dynamic signaling processes in living tissue. Classically, this approach has been constrained by limitations of lens–based and point-scanning illumination systems. Here we describe a novel microscope configuration that incorporates a nematic liquid crystal spatial light modulator (LC-SLM) to generate holographic patterns of illumination. This microscope can produce illumination spots of variable size and number and patterns shaped to precisely match user-defined elements in a specimen. Using holographic illumination to photolyse caged glutamate in brain slices, we demonstrate that shaped excitation on segments of neuronal dendrites and simultaneous, multi-spot excitation of different dendrites enables precise spatial and rapid temporal control of glutamate receptor activation. By allowing the excitation volume shape to be tailored precisely, the holographic microscope provides an extremely flexible method for activation of various photosensitive proteins and small molecules.
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DOI：
10.1523/jneurosci.2399-07.2007
发表时间：
2007-08-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
DiGregorio DA;Rothman JS;Nielsen TA;Silver RA
通讯作者：
Silver RA
影响因子：
3.8
作者：
Melville, H;Milne, GF;McGloin, D
通讯作者：
McGloin, D
影响因子：
3.8
作者：
Schmitz, CHJ;Spatz, JP;Curtis, JE
通讯作者：
Curtis, JE
影响因子：
5.3
作者：
Shepherd, GMG;Svoboda, K
通讯作者：
Svoboda, K
影响因子：
48
作者：
Shoham, S;O'Connor, DH;Wang, SSH
通讯作者：
Wang, SSH