Lipoteichoic acid from Staphylococcus aureus exacerbates respiratory disease in porcine respiratory coronavirus-infected pigs.
Lipoteichoic acid from Staphylococcus aureus exacerbates respiratory disease in porcine respiratory coronavirus-infected pigs.
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DOI：
10.1016/j.tvjl.2010.03.001
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Van Reeth K
中科院分区：
文献类型：
--
作者：
Atanasova K;Van Gucht S;Barbé F;Duchateau L;Van Reeth K
本研究的目的是评估由金黄色葡萄球菌产生的脂磷壁酸（LTA）是否会加重猪呼吸道冠状病毒（PRCV）感染猪的呼吸道疾病，如先前脂多糖所示。仔猪接种PRCV，24 h后接种S.金黄色将临床体征、肺病毒滴度、支气管肺泡灌洗液（BALF）中的炎性细胞和细胞因子与PRCV和LTA接种对照组中的动物进行比较。除1头猪外，所有PRCV-LTA接种猪均发生严重呼吸道疾病，而对照组的临床体征极轻微或不存在。PRCV-LTA和PRCV接种组的病毒滴度和肉眼可见的肺部病变相似，在LTA组中未检出。PRCV-LTA组BALF中的中性粒细胞百分比高于PRCV组。PRCV-LTA组与PRCV组BALF中IFN-γ、IL-1、IL-6、IL-12/IL-23和TNF-α水平差异无统计学意义，但PRCV-LTA组IL-6、IL-12/IL-23和IFN-γ水平高于对照组。研究结果表明，实验诱导的呼吸道疾病不是由细胞因子过度产生介导的，而是反映了特定细胞因子相互作用和/或尚未鉴定的介质的协同作用。这是第一个报告病毒和LTA之间协同相互作用增强猪呼吸道疾病严重程度的体内研究。考虑到能够产生LTA的革兰氏阳性菌通常存在于猪住宿中，因此需要进一步研究该化合物在猪呼吸道疾病综合征发展中的作用。
The objective of this study was to assess if lipoteichoic acid (LTA), produced by Staphylococcus aureus, exacerbates respiratory disease in porcine respiratory coronavirus (PRCV)-infected pigs, as has previously been shown with lipopolysaccharide. Piglets were inoculated with PRCV and 24 h later with S. aureus LTA. Clinical signs, lung virus titres, inflammatory cells and cytokines in bronchoalveolar lavage fluid (BALF) were compared with those of animals in PRCV- and LTA-inoculated control groups. All PRCV–LTA-inoculated pigs except one developed severe respiratory disease, whereas clinical signs in the control groups were minimal or absent. Virus titres and grossly visible pulmonary lesions were similar in the PRCV–LTA- and PRCV-inoculated groups and were not detected in the LTA group. Neutrophil percentages in BALF were higher in the PRCV–LTA than in the PRCV group. There was no significant difference in interferon (IFN)-γ, interleukin (IL)-1, IL-6, IL-12/IL-23 and tumour necrosis factor (TNF)-α concentrations in BALF between the PRCV–LTA and PRCV groups, but levels of IL-6, IL-12/IL-23 and IFN-γ were higher in the PRCV–LTA-inoculated than in the LTA-inoculated controls. The findings suggest that the experimentally-induced respiratory disease was not mediated by cytokine over-production, but rather reflected the concerted action of particular cytokine interactions and/or as yet unidentified mediators. This is the first in vivo study to report the synergistic interaction between a virus and LTA in enhancing the severity of respiratory disease in the pig. Given that Gram-positive bacteria, capable of producing LTA, are commonly found in pig accommodation, the role of this compound in the development of the porcine respiratory disease complex requires further investigation.
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金黄色葡萄球菌中的合成脂肪酸是细胞因子释放的有效刺激。
影响因子：
15.3
作者：
Morath, Siegfried;Stadelmaier, Andreas;Geyer, Armin;Schmidt, Richard R;Hartung, Thomas
通讯作者：
Hartung, Thomas
影响因子：
--
作者：
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通讯作者：
von Aulock, Sonja
影响因子：
5.6
作者：
Medan, D;Wang, LY;Rojanasakul, Y
通讯作者：
Rojanasakul, Y
影响因子：
4.4
作者：
Rainard, Pascal;Fromageau, Angelina;Gilbert, Florence B.
通讯作者：
Gilbert, Florence B.
影响因子：
6.4
作者：
Talreja, J;Kabir, MH;Dileepan, KN
通讯作者：
Dileepan, KN