A rapid urine test for early detection of kidney injury.
A rapid urine test for early detection of kidney injury.
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DOI：
10.1038/ki.2009.96
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发表时间：
2009-07
影响因子：
19.6
通讯作者：
Bonventre, Joseph V.
中科院分区：
文献类型：
--
作者：
Vaidya, Vishal S.;Ford, Glen M.;Waikar, Sushrut S.;Wang, Yizhuo;Clement, Matthew B.;Ramirez, Victoria;Glaab, Warren E.;Troth, Sean P.;Sistare, Frank D.;Prozialeck, Walter C.;Edwards, Joshua R.;Bobadilla, Norma A.;Mefferd, Stephen C.;Bonventre, Joseph V.
肾损伤分子-1 （Kim-1）已通过美国食品和药物管理局和欧洲药品管理局的认证，作为一种高度敏感和特异性的尿液生物标志物，可在临床前研究和临床试验中监测药物性肾损伤。在这里，我们报告了一种快速直接免疫层析横向流动15分钟检测尿Kim-1（大鼠）或Kim-1（人）的方法的开发和评估。使用大鼠Kim-1试纸的尿Kim-1带强度与Kim-1水平显著相关，这是通过基于微珠的测定、组织病理学损伤和肾Kim-1的免疫组织化学评估以剂量和时间依赖的方式测量的。在镉、庆大霉素或双侧肾缺血再灌注诱导大鼠肾损伤后检测到Kim-1。在人类中，急性肾损伤患者尿液中的KIM-1条带强度明显高于健康志愿者尿液中的KIM-1条带强度。在2例恶性间皮瘤细胞减少手术后并发急性肾损伤的患者中，术中局部给予顺铂，KIM-1量尺也可以对患者的肾损伤和恢复情况进行时间评估。我们希望未来更广泛的研究将证实这些结果的实用性，这些结果表明，Kim-1/ Kim-1试纸可以灵敏准确地检测Kim-1/ Kim-1，从而为肾脏损伤提供快速诊断方法，促进临床前和临床研究中肾脏损伤的快速和早期检测。
Kidney injury molecule-1 (Kim-1) has been qualified by the Food and Drug Administration and European Medicines Agency as a highly sensitive and specific urinary biomarker to monitor drug-induced kidney injury in preclinical studies and on a case-by-case basis in clinical trials. Here we report the development and evaluation of a rapid direct immunochromatographic lateral flow 15-min assay for detection of urinary Kim-1 (rat) or KIM-1 (human). The urinary Kim-1 band intensity using the rat Kim-1 dipstick significantly correlated with levels of Kim-1 as measured by a microbead-based assay, histopathological damage, and immunohistochemical assessment of renal Kim-1 in a dose- and time-dependent manner. Kim-1 was detected following kidney injury induced in rats by cadmium, gentamicin, or bilateral renal ischemia/reperfusion. In humans, the urinary KIM-1 band intensity was significantly greater in urine from patients with acute kidney injury than in urine from healthy volunteers. The KIM-1 dipstick also enabled temporal evaluation of kidney injury and recovery in two patients who developed postoperative acute kidney injury following cytoreductive surgery for malignant mesothelioma with intraoperative local cisplatin administration. We hope that future, more extensive studies will confirm the utility of these results, which show that the Kim-1/KIM-1 dipsticks can provide a sensitive and accurate detection of Kim-1/KIM-1, thereby providing a rapid diagnostic assay for kidney damage and facilitating the rapid and early detection of kidney injury in preclinical and clinical studies.
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影响因子：
120.7
作者：
Uchino, S;Kellum, JA;Ronco, C
通讯作者：
Ronco, C
影响因子：
19.6
作者：
Han, WK;Bailly, V;Bonventre, JV
通讯作者：
Bonventre, JV
影响因子：
19.6
作者：
Prozialeck, W. C.;Vaidya, V. S.;Bonventre, J. V.
通讯作者：
Bonventre, J. V.
影响因子：
2.2
作者：
Carson, RT;Vignali, DAA
通讯作者：
Vignali, DAA
影响因子：
13.6
作者：
Gilbertson, DT;Liu, JN;Collins, AJ
通讯作者：
Collins, AJ