Expression and role of leptin under hypoxic conditions in human testis: organotypic in vitro culture experiment and clinical study on patients with varicocele.
Expression and role of leptin under hypoxic conditions in human testis: organotypic in vitro culture experiment and clinical study on patients with varicocele.
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缺氧条件下人睾丸瘦素的表达及作用：精索静脉曲张患者的器官型体外培养实验及临床研究。
DOI：
10.1016/j.juro.2014.06.072
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Bin Chen
中科院分区：
文献类型：
--
作者：
K. Ni;K. Steger;Hao Yang;Hongxiang Wang;K. Hu;Bin Chen
目的探讨低氧条件下瘦素在人睾丸组织中的表达及其作用。材料与方法以前列腺癌患者睾丸切除后的5个睾丸为材料，建立体外低氧培养体系。免疫组织化学方法检测瘦素蛋白的表达。用定量逆转录聚合酶链式反应检测瘦素、瘦素受体和缺氧诱导因子-1α的表达。对10例健康精索静脉曲张患者和42例精索静脉曲张不育患者手术前后血清和精浆瘦素、性腺激素和精液参数进行检测。结果体外培养的精索静脉曲张患者睾丸组织的形态分析、细胞计数和细胞特异性mRNAs检测结果显示，体外培养的睾丸组织在48h内存活良好。免疫组织化学结果显示，瘦素主要在生精小管表达。在低氧条件下，Leptin、Leptin m RNA和H IF-1αm RNA的光密度显著增加。瘦素和HIF-1α基因表达呈正相关(Rs=0.843.0 5，P<0.0 1)。在临床研究中，精索静脉曲张患者术前精浆瘦素浓度明显高于对照组(平均值±SD3.01±1.23 ng/L，P<0.01)。3级组最高(3.95±1.37 ng/L，P<0.01)，术后6个月组显著下降(2.35±0.78，P<0.05)。此外，精浆瘦素水平与精子密度(Rs=−0.187，p<；0.0 5)和进行性活动率(Rs=−0.234，p<；0.0 5)呈负相关。结论人睾丸在低氧条件下表达Leptin，可能是通过HIF-1α相关反应途径实现的。精浆瘦素与精索静脉曲张相关生精功能障碍密切相关。它可以有效地反映睾丸的低氧环境。
PurposeWe investigated the expression and the exact role of leptin under hypoxic conditions in the human testis.Materials and MethodsFive testes from patients treated with orchiectomy for prostate cancer were used to construct an in vitro hypoxic culture system for human testicular tissue. Immunohistochemistry was performed to analyze leptin protein expression. Leptin, leptin receptor and HIF-1α mRNA were examined by quantitative reverse transcriptase-polymerase chain reaction. Serum and seminal plasma leptin, gonadal hormones and semen parameters were evaluated in 10 healthy donors and 42 infertile patients with varicocele before and after surgery.ResultsThe viability of in vitro cultured testicular tissue was well maintained within 48 hours based on the results of morphological analysis, cell number and cell specific mRNAs. Immunohistochemistry demonstrated that leptin was mainly expressed in seminiferous tubules. Interestingly the optical density of leptin, leptin mRNA and HIF-1α mRNA was significantly increased under hypoxia. Leptin mRNA and HIF-1α mRNA correlated positively (Rs= 0.843, p <0.01). In the clinical study the concentration of seminal plasma leptin before varicocelectomy was markedly higher in patients (mean ± SD 3.01 ± 1.23 ng/l, p <0.01). It was highest in the grade 3 group (mean 3.95 ± 1.37 ng/l, p <0.01) and significantly decreased in the 6-month postoperative group (2.35 ± 0.78, p <0.05). Furthermore, negative correlations were observed between seminal plasma leptin and the sperm concentration (Rs= −0.187, p <0.05), and progressive motility (Rs= −0.234, p <0.05).ConclusionsLeptin expression was induced under hypoxia in the human testis, probably via the HIF-1α related response pathway. Seminal plasma leptin closely correlated with varicocele related spermatogenesis dysfunction. It might effectively reflect the testicular hypoxic environment.
影响因子：
4
作者：
Cayli, S;Sakkas, D;Huszar, G
通讯作者：
Huszar, G