Formin homology 2 domains occur in multiple contexts in angiosperms.
Formin homology 2 domains occur in multiple contexts in angiosperms.
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DOI：
10.1186/1471-2164-5-44
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发表时间：
2004-07-15
期刊：
影响因子：
4.4
通讯作者：
Zárský V
中科院分区：
文献类型：
--
作者：
Cvrcková F;Novotný M;Pícková D;Zárský V
在真核细胞形态发生的广泛多样的过程中，保守的分子模块和细胞机制的参与导致了一个有趣的问题：相似的蛋白质如何有助于不同的形态发生输出。形成蛋白（FH 2蛋白）在肌动蛋白组织和动力学的控制中起着核心作用，提供了一个很好的例子，在各种谱系特异性结构和信号相互作用的背景下，进化上通用的保守蛋白质结构域的使用。为了确定FH 2蛋白家族中可能的植物特异性序列特征，我们对公共数据库中的被子植物形成蛋白相关序列进行了详细分析，特别关注完整的拟南芥基因组和接近完成的水稻基因组序列。这导致了对22个拟南芥formin相关基因中的一半的当前注释的修订。两种植物基因组的比较分析揭示了先前描述的两个植物formins亚科（I类和II类）以及其中的几个亚科（似乎早于单子叶植物和双子叶植物的分离）的良好保守性。此外，许多植物II类formin共享一个额外的保守结构域，相关的蛋白磷酸酶/张力蛋白/生长素折叠。然而，相当大的物种间的变异性限制了任何功能的结论，如拟南芥单一物种的推广。保守的FH 2结构域的植物特异性结构域背景，以及结构域本身的植物特异性特征，可以反映植物细胞中不同的功能要求。在植物中发现的纤维结构的变异性远远超过了已知的真菌和后生动物，这表明FH 2蛋白在多细胞植物类型的进化中可能有贡献。
Involvement of conservative molecular modules and cellular mechanisms in the widely diversified processes of eukaryotic cell morphogenesis leads to the intriguing question: how do similar proteins contribute to dissimilar morphogenetic outputs. Formins (FH2 proteins) play a central part in the control of actin organization and dynamics, providing a good example of evolutionarily versatile use of a conserved protein domain in the context of a variety of lineage-specific structural and signalling interactions. In order to identify possible plant-specific sequence features within the FH2 protein family, we performed a detailed analysis of angiosperm formin-related sequences available in public databases, with particular focus on the complete Arabidopsis genome and the nearly finished rice genome sequence. This has led to revision of the current annotation of half of the 22 Arabidopsis formin-related genes. Comparative analysis of the two plant genomes revealed a good conservation of the previously described two subfamilies of plant formins (Class I and Class II), as well as several subfamilies within them that appear to predate the separation of monocot and dicot plants. Moreover, a number of plant Class II formins share an additional conserved domain, related to the protein phosphatase/tensin/auxilin fold. However, considerable inter-species variability sets limits to generalization of any functional conclusions reached on a single species such as Arabidopsis. The plant-specific domain context of the conserved FH2 domain, as well as plant-specific features of the domain itself, may reflect distinct functional requirements in plant cells. The variability of formin structures found in plants far exceeds that known from both fungi and metazoans, suggesting a possible contribution of FH2 proteins in the evolution of the plant type of multicellularity.
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影响因子：
3.3
作者：
Fujiwara, T;Tanaka, K;Takai, Y
通讯作者：
Takai, Y
影响因子：
30.8
作者：
GISH, W;STATES, DJ
通讯作者：
STATES, DJ
影响因子：
14.9
作者：
Deutsch, M;Long, M
通讯作者：
Long, M
DOI：
10.1002/prot.340230315
发表时间：
1995-11-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
CHINEA, G;PADRON, G;VRIEND, G
通讯作者：
VRIEND, G
影响因子：
2.5
作者：
DELAPOMPA, JL;JAMES, D;ZELLER, R
通讯作者：
ZELLER, R