Synthesis of bone-like nanocomposites using multiphosphorylated peptides.
Synthesis of bone-like nanocomposites using multiphosphorylated peptides.
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DOI：
10.1016/j.actbio.2014.01.007
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发表时间：
2014-05
影响因子：
9.7
通讯作者：
Beniash, Elia
中科院分区：
文献类型：
--
作者：
Sfeir, Charles;Fang, Ping-An;Jayaraman, Thottala;Raman, Aparna;Zhang Xiaoyuan;Beniash, Elia
对于用于矿化组织修复和再生的新型材料存在巨大需求。这种组织的两个例子，骨和牙本质，是高度组织化的分层纳米复合材料，其中矿物和有机相在分子水平上界面。相比之下，目前的移植材料要么是陶瓷粉末，要么是矿物和有机相的物理混合物，其机械性能远远低于其靶组织的机械性能。本研究的目的是合成具有高度集成的有机/无机相的复合纳米纤维，其灵感来自骨和牙本质的矿化胶原纤维。利用我们对骨和牙本质生物矿化的理解，我们首先基于牙本质和牙槽骨中发现的高度磷酸化的蛋白质牙本质磷酸蛋白（DPP）设计了含有3个Ser-Ser-Asp重复基序的生物启发肽。我们证明，高达80%的丝氨酸的肽可以磷酸化酪蛋白激酶。我们进一步测试了这些肽诱导胶原纤维的仿生磷酸钙矿化的能力。我们的矿化研究表明，在这些磷酸化肽的存在下，形成了结构上类似于骨和牙本质的矿化胶原纤维的矿化胶原纤维。我们的研究结果表明，使用磷酸化的DPP启发的肽，我们可以成功地合成具有集成的有机和无机相的仿生复合纳米纤维。这些结果提供了第一步的仿生纳米结构材料的矿化组织修复和再生使用磷酸肽的发展。
There is a great need for novel materials for mineralized tissue repair and regeneration. Two examples of such tissue, bone and dentin, are highly organized hierarchical nanocomposites in which mineral and organic phases interface at the molecular level. In contrast, current graft materials are either ceramic powders or physical blends of mineral and organic phases with mechanical properties far inferior to those of their target tissues. The objective of this study was to synthesize composite nanofibrils with highly integrated organic/inorganic phases inspired by the mineralized collagen fibrils of bone and dentin. Utilizing our understanding of bone and dentin biomineralization, we have first designed bioinspired peptides containing 3 Ser-Ser-Asp repeat motifs based on the highly phosphorylated protein, dentin phosphophoryn (DPP) found in dentin and alveolar bone. We demonstrate that up to 80% of serines in the peptide can be phosphorylated by casein kinases. We further tested the ability of these peptides to induce biomimetic calcium phosphate mineralization of collagen fibrils. Our mineralization studies have revealed that in the presence of these phosphorylated peptides, mineralized collagen fibrils structurally similar to the mineralized collagen fibrils of bone and dentin were formed. Our results demonstrate that using phosphorylated DPP-inspired peptides, we can successfully synthesize biomimetic composite nanofibrils with integrated organic and inorganic phases. These results provide the first step in the development of biomimetic nanostructured materials for mineralized tissue repair and regeneration using phosphopeptides.
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影响因子：
3.8
作者：
Deshpande, Atul S.;Beniash, Elia
通讯作者：
Beniash, Elia
影响因子：
6.2
作者：
Deshpande, Atul Suresh;Fang, Ping-An;Zhang, Xiaoyuan;Jayaraman, Thottala;Sfeir, Charles;Beniash, Elia
通讯作者：
Beniash, Elia
影响因子：
4.8
作者：
Jadlowiec, J;Koch, H;Sfeir, C
通讯作者：
Sfeir, C
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS