Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes.
Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes.
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葡萄3-羟基-3-甲基戊二酰辅酶A还原酶（VvHMGR）在不同葡萄基因型中的表达多样性
DOI：
10.1186/s12870-021-03073-8
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发表时间：
2021-06-19
影响因子：
5.3
通讯作者：
Fang J
中科院分区：
文献类型：
--
作者：
Zheng T;Guan L;Yu K;Haider MS;Nasim M;Liu Z;Li T;Zhang K;Jiu S;Jia H;Fang J
研究背景3-羟基-3-甲基戊二酰辅酶A还原酶（3-Hydroxy-3-methylglutaryl-CoA reductase，HMGR）是甲羟戊酸（MVA）代谢途径中的关键酶之一，参与调节细胞质中萜类化合物的代谢，并决定下游萜类化合物代谢产物的类型和含量。“巨峰”果实中VvHMGR的表达具有组织特异性，随着果实发育，VvHMGR 1的表达量一直保持较高水平，VvHMGR 2的表达量最低，VvHMGR 3的表达量逐渐降低; VvHMGR 3与CsHMGR 1和GmHMGR 9关系密切，与CsHMGR 2和GmHMGR 4共线性。通过相互作用蛋白预测，VvHMGR 3与HMG-CoA合成酶、MVA激酶、FPP/GGPP合成酶、二磷酸甲羟戊酸脱羧酶等相互作用，参与萜类化合物的合成和代谢; VvHMGR 3与类胡萝卜素生物合成和MEP途径中的一些基因表达趋势相似; VvHMGR 3对各种环境和植物激素刺激有响应，尤其是盐胁迫和紫外线（UV）处理。VvHMGR在不同色香葡萄中的表达量存在差异，黄色葡萄品种中VvHMGR的表达量显著高于红色葡萄品种，玫瑰香葡萄品种中VvHMGR的表达量显著高于草莓香葡萄。结论该研究证实了VvHMGR 3在葡萄果实着色和香气形成过程中的重要作用，为解释生产过程中葡萄香气丧失和着色不良提供了新的思路。在HMGR表达方面，颜色和香气之间可能存在累加效应。
Background3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a key enzyme in the mevalonate (MVA) pathway, which regulates the metabolism of terpenoids in the cytoplasm and determines the type and content of downstream terpenoid metabolites.ResultsResults showed that grapevine HMGR family has three members, such asVvHMGR1,VvHMGR2, andVvHMGR3. The expression ofVvHMGRsin 'Kyoho' has tissue specificity, for example,VvHMGR1keeps a higher expression,VvHMGR2is the lowest, andVvHMGR3gradually decreases as the fruit development.VvHMGR3is closely related toCsHMGR1andGmHMGR9and has collinearity withCsHMGR2andGmHMGR4. By the prediction of interaction protein, it can interact with HMG-CoA synthase, MVA kinase, FPP/GGPP synthase, diphosphate mevalonate decarboxylase, and participates in the synthesis and metabolism of terpenoids.VvHMGR3have similar trends in expression with some of the genes of carotenoid biosynthesis and MEP pathways.VvHMGR3responds to various environmental and phytohormone stimuli, especially salt stress and ultraviolet (UV) treatment. The expression level ofVvHMGRsis diverse in grapes of different colors and aroma.VvHMGRsare significantly higher in yellow varieties than that in red varieties, whereas rose-scented varieties showed significantly higher expression than that of strawberry aroma. The expression level is highest in yellow rose-scented varieties, and the lowest in red strawberry scent varieties, especially ‘Summer Black’ and ‘Fujiminori’.ConclusionThis study confirms the important role ofVvHMGR3in the process of grape fruit coloring and aroma formation, and provided a new idea to explain the loss of grape aroma and poor coloring during production. There may be an additive effect between color and aroma in the HMGR expression aspect.
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影响因子：
6.2
作者：
Alam, Pravej;Abdin, M. Z.
通讯作者：
Abdin, M. Z.
影响因子：
2.8
作者：
Darabi, Maryam;Masoudi-Nejad, Ali;Nemat-Zadeh, Ghorbanali
通讯作者：
Nemat-Zadeh, Ghorbanali
影响因子：
13.8
作者：
Enfissi, EMA;Fraser, PD;Bramley, PM
通讯作者：
Bramley, PM
影响因子：
11.6
作者：
CHOI, D;WARD, BL;BOSTOCK, RM
通讯作者：
BOSTOCK, RM
影响因子：
12.3
作者：
Friesen JA;Rodwell VW
通讯作者：
Rodwell VW