Quantitative infrared photothermal microscopy
Quantitative infrared photothermal microscopy
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定量红外光热显微镜
DOI:
10.1117/12.2545159
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发表时间:
2020
期刊:
影响因子:
--
通讯作者:
Kuno, Masaru K.
中科院分区:
文献类型:
--
作者:
Pavlovetc, Ilia M.;Podshivaylov, Eduard A.;Frantsuzov, Pavel A.;Hartland, Gregory V.;Kuno, Masaru K.
Infrared photothermal heterodyne imaging (IR-PHI) represents a convenient, table top approach for conducting super-resolution imaging and spectroscopy throughout the all-important mid infrared (MIR) spectral region. Although IR-PHI provides label-free, super-resolution MIR absorption information, it is not quantitative. In this study, we establish quantitative relationships between observed IR-PHI signals and relevant photothermal parameters of investigated specimens. Specifically, we conduct a size series analysis of different radii polystyrene (PS) beads to quantitatively link IR-PHI signal contrast to specimen heat capacity, thermo-optic coefficient, MIR peak absorption cross-section, and scattering cross-section at IR-PHI’s probe wavelength.
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