Fast optical transillumination tomography with large-size projection acquisition.
Fast optical transillumination tomography with large-size projection acquisition.
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DOI：
10.1007/s10439-008-9549-1
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发表时间：
2008-10
影响因子：
3.8
通讯作者：
Haidekker, Mark A.
中科院分区：
文献类型：
--
作者：
Huang, Hsuan-Ming;Xia, Jinjun;Haidekker, Mark A.
诸如光学相干断层扫描和漫射光学断层扫描之类的技术已经被证明可以有效地对诸如组织之类的高度散射样本进行成像。另一种模式受到的关注要少得多：光学透照断层扫描（OT），一种承诺体积扫描非常高的采集速度的模式。随着组织工程血管成像可能的生物力学测试的动机，我们已经开发了一种快速OT设备，使用准直，非相干光束与大直径一起与大尺寸CMOS相机，有能力在一个单一的旋转样本获取3D投影。此外，我们使用加速迭代重建技术来提高图像重建速度，同时获得比通过滤波反投影更好的图像质量。使用墨水填充的PTFE管测试该装置，以确定几何重建精度和吸光度的恢复。即使在轻微的折射率失配的存在下，测量半径的加权误差在所有情况下都小于5%，并且发现与R2 =0.99的分光计确定的油墨吸光度的高度线性相关性，尽管OT设备系统地低估了吸光度。使用我们的优化算法，重建时间从几个小时（标准算术重建）提高到90秒/切片。该设备仅由一个光源、两个空间滤波器、一个样品池和一个CMOS相机组成，非常简单，而且成本低廉。
Techniques such as optical coherence tomography and diffuse optical tomography have been shown to effectively image highly scattering samples such as tissue. An additional modality has received much less attention: Optical transillumination tomography (OT), a modality that promises very high acquisition speed for volumetric scans. With the motivation to image tissue-engineered blood vessels for possible biomechanical testing, we have developed a fast OT device using a collimated, non-coherent beam with a large diameter together with a large-size CMOS camera that has the ability to acquire 3D projections in a single revolution of the sample. In addition, we used accelerated iterative reconstruction techniques to improve image reconstruction speed, while at the same time obtaining better image quality than through filtered back projection. The device was tested using ink-filled PTFE tubes to determine geometric reconstruction accuracy and recovery of absorbance. Even in the presence of minor refractive index mismatch, the weighted error of the measured radius was less than 5% in all cases, and a high linear correlation of ink absorbance determined with a photospectrometer of R 2 =0.99 was found, although the OT device systematically underestimated absorbance. Reconstruction time was improved from several hours (standard arithmetic reconstruction) to 90 seconds per slice with our optimized algorithm. Composed of only a light source, two spatial filters, a sample bath, and a CMOS camera, this device was extremely simple and cost-efficient to build.
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影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
2.3
作者：
ANDERSEN, AH;KAK, AC
通讯作者：
KAK, AC
影响因子：
9.6
作者：
CHARLESWORTH, PM;BREWSTER, DC;HALLET, JW
通讯作者：
HALLET, JW
影响因子：
3.8
作者：
Gladish, JC;Yao, G;Haidekker, MA
通讯作者：
Haidekker, MA
DOI：
10.3109/10731198009118977
发表时间：
1980-01-01
期刊：
BIOMATERIALS MEDICAL DEVICES AND ARTIFICIAL ORGANS
影响因子：
--
作者：
GUIDOIN, R;NOEL, HP;BLAIS, P
通讯作者：
BLAIS, P